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Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Biophys+Chem 2021 ; 270 (ä): 106538 Nephropedia Template TP
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Recent trends in analytical and digital techniques for the detection of the SARS-Cov-2 #MMPMID33418105
Das Mukhopadhyay C; Sharma P; Sinha K; Rajarshi K
Biophys Chem 2021[Mar]; 270 (ä): 106538 PMID33418105show ga
The current global outbreak of COVID-19 due to SARS-CoV-2 is an unprecedented humanitarian crisis. Considering the gravity of its impact there is an immediate need to develop a detection technique that is sensitive, specific, fast, and affordable for the clinical diagnosis of the disease. Real time Polymerase Chain Reaction (RT-PCR)-based detection platforms are contemplated to be the gold standard to detect viral RNA. However, that may be susceptible to errors, and there is a risk of obtaining false results, which ultimately compromises the strategy of efficient disease management. Several modern techniques exhibiting assured results with enhanced sensitivity and specificity against the SARS-CoV-2 associated viral components or immune response against it have been developed and may be implemented. The review deals with the conventional RT-PCR detection techniques and compares them to other detection platforms viz., biosensor based detection of antigens, fluorescent or colorimetric detection systems including CRISPR-Cas 13 based SHERLOCK kit, CRISPR Cas-9 based FELUDA test kit, CRISPR DETECTR kit, Next Generation Sequencing or microarray-based kits. These modern techniques are great as a point of care detection methods but should be followed by RT PCR based detection for the confirmation of COVID-19 status.
|Antigens, Viral/analysis[MESH]
|Biosensing Techniques/*methods[MESH]
|COVID-19/*diagnosis/virology[MESH]
|Clustered Regularly Interspaced Short Palindromic Repeats/genetics[MESH]