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10.1002/jmv.26782 http://scihub22266oqcxt.onion/10.1002/jmv.26782 33415765!ä!33415765 Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=33415765&cmd=llinks): Failed to open stream: HTTP request failed! 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Development and implementation of a RT-qPCR extraction-free protocol for the detection of SARS-CoV-2 and impact on the turn-around-time |pdf=|usr=}}{{33415765}} gab.com Text Development and implementation of a RT-qPCR extraction-free protocol for the detection of SARS-CoV-2 and impact on the turn-around-time JO: J Med Virol http://www.kidney.de/pget.php?&tw=1&pmid=33415765 #FOAvip The occurrence of the COVID-19 second-wave outbreak in Europe has pushed laboratories performing molecular SARS-CoV-2 tests to increase their throughput and decrease the result rendering time. In this evaluation, we tested for the first time a new, extraction-free, protocol with the Allplex SARS-CoV-2 Assay RT-qPCR kit on a Nimbus platform. Ninety-one samples, of which 71 previously tested positive with RT-qPCR with extraction were immediately analyzed without extraction, using only a dilution and thermal shock protocol. The positive and negative percentage agreements were respectively 97.2% (95% confidence interval [CI]: 0.90-0.99) and 95.0% (95% CI: 0.76-0.99). The two false negatives observed were very weakly positive with the comparison method. Moderate variations in Ct of the targeted genes were observed (median +/- 95% CI): E gene, +2.49 +/- 0.44; N gene, +0.98 +/- 0.54; RdRP/S genes, +2.64 +/- 0.48. On the other hand, the number of tests performed within 24 h raised from 86.4% to 97.8%, the turn-around time decreased from 19:18 to 09:03 (p < .0001), and the number of tests that can be performed per day doubled since this technique was introduced routinely in our laboratory. Twit Text FOAVip Development and implementation of a RT-qPCR extraction-free protocol for the detection of SARS-CoV-2 and impact on the turn-around-time ????J Med Virol http://www.kidney.de/pget.php?&tw=1&pmid=33415765 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33415765 #FOAvip English Wikipedia <ref>{{Cite pmid|33415765}}</ref> Development and implementation of a RT-qPCR extraction-free protocol for the detection of SARS-CoV-2 and impact on the turn-around-time #MMPMID33415765 Blairon L; Piteus S; Beukinga I; Tre-Hardy M J Med Virol 2021[Apr]; 93 (4): 2538-2542 PMID33415765show ga The occurrence of the COVID-19 second-wave outbreak in Europe has pushed laboratories performing molecular SARS-CoV-2 tests to increase their throughput and decrease the result rendering time. In this evaluation, we tested for the first time a new, extraction-free, protocol with the Allplex SARS-CoV-2 Assay RT-qPCR kit on a Nimbus platform. Ninety-one samples, of which 71 previously tested positive with RT-qPCR with extraction were immediately analyzed without extraction, using only a dilution and thermal shock protocol. The positive and negative percentage agreements were respectively 97.2% (95% confidence interval [CI]: 0.90-0.99) and 95.0% (95% CI: 0.76-0.99). The two false negatives observed were very weakly positive with the comparison method. Moderate variations in Ct of the targeted genes were observed (median +/- 95% CI): E gene, +2.49 +/- 0.44; N gene, +0.98 +/- 0.54; RdRP/S genes, +2.64 +/- 0.48. On the other hand, the number of tests performed within 24 h raised from 86.4% to 97.8%, the turn-around time decreased from 19:18 to 09:03 (p < .0001), and the number of tests that can be performed per day doubled since this technique was introduced routinely in our laboratory. |COVID-19 Testing/*methods[MESH] |COVID-19/diagnosis/*virology[MESH] |Coronavirus Envelope Proteins/genetics[MESH] |Coronavirus Nucleocapsid Proteins/genetics[MESH] |Disease Outbreaks[MESH] |Europe[MESH] |Genes, Viral[MESH] |Humans[MESH] |Phosphoproteins/genetics[MESH] |RNA, Viral/analysis/genetics[MESH] |Real-Time Polymerase Chain Reaction/*methods[MESH] |SARS-CoV-2/genetics/*isolation & purification[MESH]Development and implementation of a RT-qPCR extraction-free protocol f(epmc).pdf DeepDyve Pubget Overpricing