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10.1016/j.jim.2021.112958 http://scihub22266oqcxt.onion/10.1016/j.jim.2021.112958 33412174!7831464!33412174     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=33412174&cmd=llinks): Failed to open stream: HTTP request failed! 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Development and characterization of an indirect ELISA to detect SARS-CoV-2 spike protein-specific antibodies |pdf=|usr=}}{{33412174}} gab.com Text Development and characterization of an indirect ELISA to detect SARS-CoV-2 spike protein-specific antibodies JO: J Immunol Methods http://www.kidney.de/pget.php?&tw=1&pmid=33412174 #FOAvip The current Severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2) pandemic is a public health emergency of international concern. Sensitive and precise diagnostic tools are urgently needed. In this study, we developed a SARS-CoV-2 spike (S1) protein enzyme-linked immunosorbent assay (ELISA) to detect SARS-CoV-2-specific antibodies. The SARS-CoV-2 S1 ELISA was found to be specific [97.8% (95% CI, 96.7% - 98.5%)], reproducible and precise (intra-assay coefficient of variability (CV) 5.3%, inter-assay CV 7.9%). A standard curve and the interpolation of arbitrary ELISA units per milliliter served to reduce the variability between different tests and operators. Cross-reactivity to other human coronaviruses was addressed by using sera positive for MERS-CoV- and hCoV HKU1-specific antibodies. Monitoring antibody development in various samples of twenty-three and single samples of twenty-nine coronavirus disease 2019 (COVID-19) patients revealed seroconversion and neutralizing antibodies against authentic SARS-CoV-2 in all cases. The comparison of the SARS-CoV-2 (S1) ELISA with a commercially available assay showed a better sensitivity for the in-house ELISA. The results demonstrate a high reproducibility, specificity and sensitivity of the newly developed ELISA, which is suitable for the detection of SARS-CoV-2 S1 protein-specific antibody responses. Twit Text FOAVip Development and characterization of an indirect ELISA to detect SARS-CoV-2 spike protein-specific antibodies ????J Immunol Methods http://www.kidney.de/pget.php?&tw=1&pmid=33412174 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33412174 #FOAvip English Wikipedia <ref>{{Cite pmid|33412174}}</ref> Development and characterization of an indirect ELISA to detect SARS-CoV-2 spike protein-specific antibodies #MMPMID33412174 Krahling V; Halwe S; Rohde C; Becker D; Berghofer S; Dahlke C; Eickmann M; Ercanoglu MS; Gieselmann L; Herwig A; Kupke A; Muller H; Neubauer-Radel P; Klein F; Keller C; Becker S J Immunol Methods 2021[Mar]; 490 (ä): 112958 PMID33412174show ga The current Severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2) pandemic is a public health emergency of international concern. Sensitive and precise diagnostic tools are urgently needed. In this study, we developed a SARS-CoV-2 spike (S1) protein enzyme-linked immunosorbent assay (ELISA) to detect SARS-CoV-2-specific antibodies. The SARS-CoV-2 S1 ELISA was found to be specific [97.8% (95% CI, 96.7% - 98.5%)], reproducible and precise (intra-assay coefficient of variability (CV) 5.3%, inter-assay CV 7.9%). A standard curve and the interpolation of arbitrary ELISA units per milliliter served to reduce the variability between different tests and operators. Cross-reactivity to other human coronaviruses was addressed by using sera positive for MERS-CoV- and hCoV HKU1-specific antibodies. Monitoring antibody development in various samples of twenty-three and single samples of twenty-nine coronavirus disease 2019 (COVID-19) patients revealed seroconversion and neutralizing antibodies against authentic SARS-CoV-2 in all cases. The comparison of the SARS-CoV-2 (S1) ELISA with a commercially available assay showed a better sensitivity for the in-house ELISA. The results demonstrate a high reproducibility, specificity and sensitivity of the newly developed ELISA, which is suitable for the detection of SARS-CoV-2 S1 protein-specific antibody responses. |Animals[MESH] |Antibodies, Neutralizing/blood[MESH] |COVID-19 Serological Testing/*methods[MESH] |COVID-19/*diagnosis[MESH] |Chlorocebus aethiops[MESH] |Enzyme Assays[MESH] |Enzyme-Linked Immunosorbent Assay[MESH] |Epithelial Cells/*metabolism[MESH] |Humans[MESH] |Reproducibility of Results[MESH] |SARS-CoV-2/*metabolism[MESH] |Sensitivity and Specificity[MESH] |Seroconversion[MESH] |Spike Glycoprotein, Coronavirus/genetics/immunology/*metabolism[MESH]Development and characterization of an indirect ELISA to detect SARS-C(epmc).pdf DeepDyve Pubget Overpricing