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10.1039/d0an01686a http://scihub22266oqcxt.onion/10.1039/d0an01686a 33410826!ä!33410826 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Analyst 2021 ; 146 (2): 382-402 Nephropedia Template TP {{tp|p=33410826|t=2021. Current advances in the detection of COVID-19 and evaluation of the humoral response |pdf=|usr=}}{{33410826}} gab.com Text Current advances in the detection of COVID-19 and evaluation of the humoral response JO: Analyst http://www.kidney.de/pget.php?&tw=1&pmid=33410826 #FOAvip The new outbreak caused by coronavirus SARS-CoV-2 started at the end of 2019 and was declared a pandemic in March 2020. Since then, several diagnostic approaches have been re-adapted, and also improved from the previous detections of SARS and MERS coronavirus. The best strategy to handle this situation seems to rely on a triad of detection methods: (i) highly sensitive and specific techniques as the gold standard method, (ii) easier and faster point of care tests accessible for large population screening, and (iii) serology assays to complement the direct detection and to use for surveillance. In this study, we assessed the techniques and tests described in the literature, their advantages and disadvantages, and the interpretation of the results. Quantitative reverse transcription polymerase chain reaction (RT-qPCR) is undoubtedly the gold standard technique utilized not only for diagnostics, but also as a standard for comparison and validation of newer approaches. Other nucleic acid amplification methods have been shown to be adequate as point of care (POC) diagnostic tests with similar performance as RT-qPCR. The analysis of seroconversion with immunotests shows the complexity of the immune response to COVID-19. The detection of anti-SARS-CoV-2 antibodies can also help to detect previously infected asymptomatic individuals with negative RT-qPCR tests. Nevertheless, more controlled serology cohort studies should be performed as soon as possible to understand the immune response to SARS-CoV-2. Twit Text FOAVip Current advances in the detection of COVID-19 and evaluation of the humoral response ????Analyst http://www.kidney.de/pget.php?&tw=1&pmid=33410826 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33410826 #FOAvip English Wikipedia <ref>{{Cite pmid|33410826}}</ref> Current advances in the detection of COVID-19 and evaluation of the humoral response #MMPMID33410826 Huergo MAC; Thanh NTK Analyst 2021[Jan]; 146 (2): 382-402 PMID33410826show ga The new outbreak caused by coronavirus SARS-CoV-2 started at the end of 2019 and was declared a pandemic in March 2020. Since then, several diagnostic approaches have been re-adapted, and also improved from the previous detections of SARS and MERS coronavirus. The best strategy to handle this situation seems to rely on a triad of detection methods: (i) highly sensitive and specific techniques as the gold standard method, (ii) easier and faster point of care tests accessible for large population screening, and (iii) serology assays to complement the direct detection and to use for surveillance. In this study, we assessed the techniques and tests described in the literature, their advantages and disadvantages, and the interpretation of the results. Quantitative reverse transcription polymerase chain reaction (RT-qPCR) is undoubtedly the gold standard technique utilized not only for diagnostics, but also as a standard for comparison and validation of newer approaches. Other nucleic acid amplification methods have been shown to be adequate as point of care (POC) diagnostic tests with similar performance as RT-qPCR. The analysis of seroconversion with immunotests shows the complexity of the immune response to COVID-19. The detection of anti-SARS-CoV-2 antibodies can also help to detect previously infected asymptomatic individuals with negative RT-qPCR tests. Nevertheless, more controlled serology cohort studies should be performed as soon as possible to understand the immune response to SARS-CoV-2. |COVID-19 Testing/*methods[MESH] |COVID-19/*diagnosis/immunology/virology[MESH] |Clinical Laboratory Techniques/methods[MESH] |Humans[MESH] |Immunity, Humoral/*immunology[MESH] |Nucleic Acid Amplification Techniques/methods[MESH] |Point-of-Care Testing/*standards[MESH]Current advances in the detection of COVID-19 and evaluation of the hu(epmc).pdf DeepDyve Pubget Overpricing