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10.3390/healthcare9010037 http://scihub22266oqcxt.onion/10.3390/healthcare9010037 33406585!7823392!33406585     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Healthcare+(Basel) 2021 ; 9 (1): ä Nephropedia Template TP {{tp|p=33406585|t=2021. A Direct Method for RT-PCR Detection of SARS-CoV-2 in Clinical Samples |pdf=|usr=}}{{33406585}} gab.com Text A Direct Method for RT-PCR Detection of SARS-CoV-2 in Clinical Samples JO: Healthcare (Basel) http://www.kidney.de/pget.php?&tw=1&pmid=33406585 #FOAvip INTRODUCTION: the emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused a global pandemic of acute respiratory disease (COVID-19). SARS-CoV-2 is a positive-strand RNA virus and its genomic characterization has played a vital role in the design of appropriate diagnostics tests. The current RT-PCR protocol for SARS-CoV-2 detects two regions of the viral genome, requiring RNA extraction and several hours. There is a need for fast, simple, and cost-effective detection strategies. METHODS: we optimized a protocol for direct RT-PCR detection of SARS-CoV-2 without the need for nucleic acid extraction. Nasopharyngeal samples were diluted to 1:3 using diethyl pyrocarbonate (DEPC)-treated water. The diluted samples were incubated at 95 degrees C for 5 min in a thermal cycler, followed by a cooling step at 4 degrees C for 5 min. Samples then underwent reverse transcription real-time RT-PCR in the E and RdRp genes. RESULTS: our direct detection protocol showed 100% concordance with the standard protocol with an average Ct value difference of 4.38 for the E region and 3.85 for the RdRp region. CONCLUSION: the direct PCR technique was found to be a reliable and sensitive method that can be used to reduce the time and cost of the assay by removing the need for RNA extraction. It enables the use of the assay in research, diagnostics, and screening for COVID-19 in regions with fewer economic resources, where supplies are more limited allowing for wider use for screening. Twit Text FOAVip A Direct Method for RT-PCR Detection of SARS-CoV-2 in Clinical Samples ????Healthcare (Basel) http://www.kidney.de/pget.php?&tw=1&pmid=33406585 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33406585 #FOAvip English Wikipedia <ref>{{Cite pmid|33406585}}</ref> A Direct Method for RT-PCR Detection of SARS-CoV-2 in Clinical Samples #MMPMID33406585 El-Kafrawy SA; El-Daly MM; Hassan AM; Kaki RM; Abuzenadah AM; Kamal MA; Azhar EI Healthcare (Basel) 2021[Jan]; 9 (1): ä PMID33406585show ga INTRODUCTION: the emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused a global pandemic of acute respiratory disease (COVID-19). SARS-CoV-2 is a positive-strand RNA virus and its genomic characterization has played a vital role in the design of appropriate diagnostics tests. The current RT-PCR protocol for SARS-CoV-2 detects two regions of the viral genome, requiring RNA extraction and several hours. There is a need for fast, simple, and cost-effective detection strategies. METHODS: we optimized a protocol for direct RT-PCR detection of SARS-CoV-2 without the need for nucleic acid extraction. Nasopharyngeal samples were diluted to 1:3 using diethyl pyrocarbonate (DEPC)-treated water. The diluted samples were incubated at 95 degrees C for 5 min in a thermal cycler, followed by a cooling step at 4 degrees C for 5 min. Samples then underwent reverse transcription real-time RT-PCR in the E and RdRp genes. RESULTS: our direct detection protocol showed 100% concordance with the standard protocol with an average Ct value difference of 4.38 for the E region and 3.85 for the RdRp region. CONCLUSION: the direct PCR technique was found to be a reliable and sensitive method that can be used to reduce the time and cost of the assay by removing the need for RNA extraction. It enables the use of the assay in research, diagnostics, and screening for COVID-19 in regions with fewer economic resources, where supplies are more limited allowing for wider use for screening. äA Direct Method for RT-PCR Detection of SARS-CoV-2 in Clinical Samples(epmc).pdf DeepDyve Pubget Overpricing