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10.1007/s10096-020-04128-8

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suck abstract from ncbi


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pmid33404891      Eur+J+Clin+Microbiol+Infect+Dis 2021 ; 40 (3): 485-494
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  • Inference of SARS-CoV-2 spike-binding neutralizing antibody titers in sera from hospitalized COVID-19 patients by using commercial enzyme and chemiluminescent immunoassays #MMPMID33404891
  • Valdivia A; Torres I; Latorre V; Frances-Gomez C; Albert E; Gozalbo-Rovira R; Alcaraz MJ; Buesa J; Rodriguez-Diaz J; Geller R; Navarro D
  • Eur J Clin Microbiol Infect Dis 2021[Mar]; 40 (3): 485-494 PMID33404891show ga
  • Whether antibody levels measured by commercially available enzyme or chemiluminescent immunoassays targeting the SARS-CoV-2 spike (S) protein can act as a proxy for serum neutralizing activity remains to be established for many of these assays. We evaluated the degree of correlation between neutralizing antibodies (NtAb) binding the SARS-CoV-2 spike (S) protein and SARS-CoV-2-S-IgG levels measured by four commercial immunoassays in sera drawn from hospitalized COVID-19 patients. Ninety sera from 51 hospitalized COVID-19 patients were tested by a pseudotyped virus neutralization assay, the LIAISON SARS-CoV-2 S1/S2 IgG, the Euroimmun SARS-CoV-2 IgG ELISA, the MAGLUMI 2019-nCoV IgG, and the COVID-19 ELISA IgG assays. Overall, the results obtained with the COVID-19 ELISA IgG test showed the highest agreement with the NtAb assay (kappa, 0.85; 95% CI, 0.63-1). The most sensitive tests were the pseudotyped virus NtAb assay and the COVID-19 ELISA IgG assay (92.2% for both). Overall, the degree correlation between antibody titers resulting in 50% virus neutralization (NtAb(50)) in the pseudotyped virus assay and SARS-CoV-2 IgG levels was strong for the Euroimmun SARS-CoV-2 IgG ELISA (rho = 0.73) and moderate for the remaining assays (rho = 0.48 to 0.59). The kinetic profile of serum NtAb(50) titers could not be reliably predicted by any of the SARS-CoV-2 IgG immunoassays. The suitability of SARS-CoV-2-S-IgG commercial immunoassays for inferring neutralizing activity of sera from hospitalized COVID-19 patients varies widely across tests and is influenced by the time of sera collection after the onset of symptoms.
  • |Adult[MESH]
  • |Aged[MESH]
  • |Antibodies, Neutralizing/*blood[MESH]
  • |Antibodies, Viral/blood[MESH]
  • |COVID-19 Serological Testing/*methods[MESH]
  • |COVID-19/blood/*diagnosis[MESH]
  • |Female[MESH]
  • |Hospitalization[MESH]
  • |Humans[MESH]
  • |Immunoassay/*methods[MESH]
  • |Immunoglobulin G/blood[MESH]
  • |Kinetics[MESH]
  • |Male[MESH]
  • |Middle Aged[MESH]
  • |Neutralization Tests[MESH]
  • |SARS-CoV-2/*immunology[MESH]
  • |Sensitivity and Specificity[MESH]


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