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10.1038/s41594-020-00547-5 http://scihub22266oqcxt.onion/10.1038/s41594-020-00547-5 33402708!7878407!33402708     free     free     free       Nat+Struct+Mol+Biol 2021 ; 28 (2): 128-131 Nephropedia Template TP {{tp|p=33402708|t=2021. Cold sensitivity of the SARS-CoV-2 spike ectodomain |pdf=|usr=}}{{33402708}} gab.com Text Cold sensitivity of the SARS-CoV-2 spike ectodomain JO: Nat Struct Mol Biol http://www.kidney.de/pget.php?&tw=1&pmid=33402708 #FOAvip The SARS-CoV-2 spike (S) protein, a primary target for COVID-19 vaccine development, presents its receptor binding domain in two conformations, the receptor-accessible 'up' or receptor-inaccessible 'down' states. Here we report that the commonly used stabilized S ectodomain construct '2P' is sensitive to cold temperatures, and this cold sensitivity is abrogated in a 'down' state-stabilized ectodomain. Our findings will impact structural, functional and vaccine studies that use the SARS-CoV-2 S ectodomain. Twit Text FOAVip Cold sensitivity of the SARS-CoV-2 spike ectodomain ????Nat Struct Mol Biol http://www.kidney.de/pget.php?&tw=1&pmid=33402708 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33402708 #FOAvip English Wikipedia <ref>{{Cite pmid|33402708}}</ref> Cold sensitivity of the SARS-CoV-2 spike ectodomain #MMPMID33402708 Edwards RJ; Mansouri K; Stalls V; Manne K; Watts B; Parks R; Janowska K; Gobeil SMC; Kopp M; Li D; Lu X; Mu Z; Deyton M; Oguin TH 3rd; Sprenz J; Williams W; Saunders KO; Montefiori D; Sempowski GD; Henderson R; Munir Alam S; Haynes BF; Acharya P Nat Struct Mol Biol 2021[Feb]; 28 (2): 128-131 PMID33402708show ga The SARS-CoV-2 spike (S) protein, a primary target for COVID-19 vaccine development, presents its receptor binding domain in two conformations, the receptor-accessible 'up' or receptor-inaccessible 'down' states. Here we report that the commonly used stabilized S ectodomain construct '2P' is sensitive to cold temperatures, and this cold sensitivity is abrogated in a 'down' state-stabilized ectodomain. Our findings will impact structural, functional and vaccine studies that use the SARS-CoV-2 S ectodomain. |Antibodies, Viral/chemistry[MESH] |COVID-19 Vaccines/chemistry[MESH] |Cold Temperature[MESH] |Cryoelectron Microscopy[MESH] |Enzyme-Linked Immunosorbent Assay[MESH] |Humans[MESH] |Protein Denaturation[MESH] |Protein Domains[MESH] |Protein Stability[MESH] |Spike Glycoprotein, Coronavirus/*chemistry/ultrastructure[MESH]Cold sensitivity of the SARS-CoV-2 spike ectodomain (epmc).pdf DeepDyve Pubget Overpricing