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10.7554/eLife.64508 http://scihub22266oqcxt.onion/10.7554/eLife.64508 33393462!7806259!33393462     free     free     free Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 263.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 263.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 263.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Elife 2021 ; 10 (ä): ä Nephropedia Template TP {{tp|p=33393462|t=2021. SARS-CoV-2 entry into human airway organoids is serine protease-mediated and facilitated by the multibasic cleavage site |pdf=|usr=}}{{33393462}} gab.com Text SARS-CoV-2 entry into human airway organoids is serine protease-mediated and facilitated by the multibasic cleavage site JO: Elife http://www.kidney.de/pget.php?&tw=1&pmid=33393462 #FOAvip Coronavirus entry is mediated by the spike protein that binds the receptor and mediates fusion after cleavage by host proteases. The proteases that mediate entry differ between cell lines, and it is currently unclear which proteases are relevant in vivo. A remarkable feature of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike is the presence of a multibasic cleavage site (MBCS), which is absent in the SARS-CoV spike. Here, we report that the SARS-CoV-2 spike MBCS increases infectivity on human airway organoids (hAOs). Compared with SARS-CoV, SARS-CoV-2 entered faster into Calu-3 cells and, more frequently, formed syncytia in hAOs. Moreover, the MBCS increased entry speed and plasma membrane serine protease usage relative to cathepsin-mediated endosomal entry. Blocking serine proteases, but not cathepsins, effectively inhibited SARS-CoV-2 entry and replication in hAOs. Our findings demonstrate that SARS-CoV-2 enters relevant airway cells using serine proteases, and suggest that the MBCS is an adaptation to this viral entry strategy. Twit Text FOAVip SARS-CoV-2 entry into human airway organoids is serine protease-mediated and facilitated by the multibasic cleavage site ????Elife http://www.kidney.de/pget.php?&tw=1&pmid=33393462 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33393462 #FOAvip English Wikipedia <ref>{{Cite pmid|33393462}}</ref> SARS-CoV-2 entry into human airway organoids is serine protease-mediated and facilitated by the multibasic cleavage site #MMPMID33393462 Mykytyn AZ; Breugem TI; Riesebosch S; Schipper D; van den Doel PB; Rottier RJ; Lamers MM; Haagmans BL Elife 2021[Jan]; 10 (ä): ä PMID33393462show ga Coronavirus entry is mediated by the spike protein that binds the receptor and mediates fusion after cleavage by host proteases. The proteases that mediate entry differ between cell lines, and it is currently unclear which proteases are relevant in vivo. A remarkable feature of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike is the presence of a multibasic cleavage site (MBCS), which is absent in the SARS-CoV spike. Here, we report that the SARS-CoV-2 spike MBCS increases infectivity on human airway organoids (hAOs). Compared with SARS-CoV, SARS-CoV-2 entered faster into Calu-3 cells and, more frequently, formed syncytia in hAOs. Moreover, the MBCS increased entry speed and plasma membrane serine protease usage relative to cathepsin-mediated endosomal entry. Blocking serine proteases, but not cathepsins, effectively inhibited SARS-CoV-2 entry and replication in hAOs. Our findings demonstrate that SARS-CoV-2 enters relevant airway cells using serine proteases, and suggest that the MBCS is an adaptation to this viral entry strategy. |*Virus Internalization[MESH] |Amino Acid Motifs[MESH] |Animals[MESH] |COVID-19/virology[MESH] |Cell Fusion[MESH] |Cell Line, Tumor[MESH] |Chlorocebus aethiops[MESH] |Humans[MESH] |Organoids/*virology[MESH] |Respiratory System/*virology[MESH] |SARS-CoV-2/chemistry/*physiology[MESH] |Serine Endopeptidases[MESH] |Severe acute respiratory syndrome-related coronavirus/chemistry/physiology[MESH] |Spike Glycoprotein, Coronavirus/*chemistry[MESH]SARS-CoV-2 entry into human airway organoids is serine protease-mediat(epmc).pdf DeepDyve Pubget Overpricing