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10.7150/thno.51479 http://scihub22266oqcxt.onion/10.7150/thno.51479 33391497!7738867!33391497     free     free     free Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Theranostics 2021 ; 11 (2): 649-664 Nephropedia Template TP {{tp|p=33391497|t=2021. Rapid design and development of CRISPR-Cas13a targeting SARS-CoV-2 spike protein |pdf=|usr=}}{{33391497}} gab.com Text Rapid design and development of CRISPR-Cas13a targeting SARS-CoV-2 spike protein JO: Theranostics http://www.kidney.de/pget.php?&tw=1&pmid=33391497 #FOAvip The novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused a worldwide epidemic of the lethal respiratory coronavirus disease (COVID-19), necessitating urgent development of specific and effective therapeutic tools. Among several therapeutic targets of coronaviruses, the spike protein is of great significance due to its key role in host invasion. Here, we report a potential anti-SARS-CoV-2 strategy based on the CRISPR-Cas13a system. Methods: A comprehensive set of bioinformatics methods, including sequence alignment, structural comparison, and molecular docking, was utilized to identify a SARS-CoV-2-spike(S)-specific segment. A tiling crRNA library targeting this specific RNA segment was designed, and optimal crRNA candidates were selected using in-silico methods. The efficiencies of the crRNA candidates were tested in human HepG2 and AT2 cells. Results: The most effective crRNA sequence inducing a robust cleavage effect on S and a potent collateral cleavage effect were identified. Conclusions: This study provides a rapid design pipeline for a CRISPR-Cas13a-based antiviral tool against SARS-CoV-2. Moreover, it offers a novel approach for anti-virus study even if the precise structures of viral proteins are indeterminate. Twit Text FOAVip Rapid design and development of CRISPR-Cas13a targeting SARS-CoV-2 spike protein ????Theranostics http://www.kidney.de/pget.php?&tw=1&pmid=33391497 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33391497 #FOAvip English Wikipedia <ref>{{Cite pmid|33391497}}</ref> Rapid design and development of CRISPR-Cas13a targeting SARS-CoV-2 spike protein #MMPMID33391497 Wang L; Zhou J; Wang Q; Wang Y; Kang C Theranostics 2021[]; 11 (2): 649-664 PMID33391497show ga The novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused a worldwide epidemic of the lethal respiratory coronavirus disease (COVID-19), necessitating urgent development of specific and effective therapeutic tools. Among several therapeutic targets of coronaviruses, the spike protein is of great significance due to its key role in host invasion. Here, we report a potential anti-SARS-CoV-2 strategy based on the CRISPR-Cas13a system. Methods: A comprehensive set of bioinformatics methods, including sequence alignment, structural comparison, and molecular docking, was utilized to identify a SARS-CoV-2-spike(S)-specific segment. A tiling crRNA library targeting this specific RNA segment was designed, and optimal crRNA candidates were selected using in-silico methods. The efficiencies of the crRNA candidates were tested in human HepG2 and AT2 cells. Results: The most effective crRNA sequence inducing a robust cleavage effect on S and a potent collateral cleavage effect were identified. Conclusions: This study provides a rapid design pipeline for a CRISPR-Cas13a-based antiviral tool against SARS-CoV-2. Moreover, it offers a novel approach for anti-virus study even if the precise structures of viral proteins are indeterminate. |*COVID-19 Drug Treatment[MESH] |Antiviral Agents/*administration & dosage[MESH] |COVID-19/virology[MESH] |CRISPR-Cas Systems/genetics[MESH] |Computational Biology[MESH] |Drug Evaluation, Preclinical[MESH] |Genetic Vectors/administration & dosage/genetics[MESH] |Hep G2 Cells[MESH] |Humans[MESH] |Molecular Docking Simulation[MESH] |RNA, Guide, CRISPR-Cas Systems/*genetics[MESH] |SARS-CoV-2/genetics[MESH] |Sequence Homology, Amino Acid[MESH]Rapid design and development of CRISPR-Cas13a targeting SARS-CoV-2 spi(epmc).pdf DeepDyve Pubget Overpricing