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10.1055/s-0040-1722551 http://scihub22266oqcxt.onion/10.1055/s-0040-1722551 33390676!7773443!33390676     free     free     free Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Lab+Physicians 2020 ; 12 (4): 268-270 Nephropedia Template TP {{tp|p=33390676|t=2020. Stability of SARS-CoV-2 RNA in Viral Lysis Buffer Stored at Different Temperatures |pdf=|usr=}}{{33390676}} gab.com Text Stability of SARS-CoV-2 RNA in Viral Lysis Buffer Stored at Different Temperatures JO: J Lab Physicians http://www.kidney.de/pget.php?&tw=1&pmid=33390676 #FOAvip Objectives The present COVID-19 pandemic resulted in an increased need for molecular diagnostic testing. Delay in the specimen processing and suboptimal storage of suspected samples in laboratories leads to degradation of SARS-CoV-2 viral RNA. Viral lysis bu ff ers from RNA extraction kits have the potential to stabilize RNA. Hence, this study aimed to investigate the stability of SARS-CoV-2 RNA in viral lysis buffer at different temperatures and time periods. Materials and Methods Aliquots of samples with known SARS-CoV-2 RNA were processed in viral lysis bu ff ers simultaneously, stored separately at 2 to 8 degrees C and 22 to 28 degrees C for 24 hours, 48 hours and 72 hours. SARS-CoV-2 viral RNA was extracted from each aliquot and analyzed using multiplex real-time PCR. Results SARS-CoV-2 RNA in samples placed in viral lysis buffer was stable for 48 hours at both 2 to 8 degrees C and 22 to 28 degrees C temperatures. Slight decline in the viral RNA quantity was found on aliquots tested after 48 hours of both the temperatures. Conclusions Viral lysis buffer maintains the integrity of SARS-CoV-2 RNA for up to 48 hours even at room temperature and supports delayed diagnosis with an overwhelming sample load in testing laboratories. Twit Text FOAVip Stability of SARS-CoV-2 RNA in Viral Lysis Buffer Stored at Different Temperatures ????J Lab Physicians http://www.kidney.de/pget.php?&tw=1&pmid=33390676 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33390676 #FOAvip English Wikipedia <ref>{{Cite pmid|33390676}}</ref> Stability of SARS-CoV-2 RNA in Viral Lysis Buffer Stored at Different Temperatures #MMPMID33390676 Perumal N; Jain RK; Shrivastava R; Lalwani J; Chaurasia D J Lab Physicians 2020[Dec]; 12 (4): 268-270 PMID33390676show ga Objectives The present COVID-19 pandemic resulted in an increased need for molecular diagnostic testing. Delay in the specimen processing and suboptimal storage of suspected samples in laboratories leads to degradation of SARS-CoV-2 viral RNA. Viral lysis bu ff ers from RNA extraction kits have the potential to stabilize RNA. Hence, this study aimed to investigate the stability of SARS-CoV-2 RNA in viral lysis buffer at different temperatures and time periods. Materials and Methods Aliquots of samples with known SARS-CoV-2 RNA were processed in viral lysis bu ff ers simultaneously, stored separately at 2 to 8 degrees C and 22 to 28 degrees C for 24 hours, 48 hours and 72 hours. SARS-CoV-2 viral RNA was extracted from each aliquot and analyzed using multiplex real-time PCR. Results SARS-CoV-2 RNA in samples placed in viral lysis buffer was stable for 48 hours at both 2 to 8 degrees C and 22 to 28 degrees C temperatures. Slight decline in the viral RNA quantity was found on aliquots tested after 48 hours of both the temperatures. Conclusions Viral lysis buffer maintains the integrity of SARS-CoV-2 RNA for up to 48 hours even at room temperature and supports delayed diagnosis with an overwhelming sample load in testing laboratories. äStability of SARS-CoV-2 RNA in Viral Lysis Buffer Stored at Different (epmc).pdf DeepDyve Pubget Overpricing