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10.1016/j.ijantimicag.2020.106274 http://scihub22266oqcxt.onion/10.1016/j.ijantimicag.2020.106274 33387629!7772996!33387629     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Int+J+Antimicrob+Agents 2021 ; 57 (3): 106274 Nephropedia Template TP {{tp|p=33387629|t=2021. Inhibition of the replication of SARS-CoV-2 in human cells by the FDA-approved drug chlorpromazine |pdf=|usr=}}{{33387629}} gab.com Text Inhibition of the replication of SARS-CoV-2 in human cells by the FDA-approved drug chlorpromazine JO: Int J Antimicrob Agents http://www.kidney.de/pget.php?&tw=1&pmid=33387629 #FOAvip INTRODUCTION: Urgent action is needed to fight the ongoing coronavirus disease 2019 (COVID-19) pandemic by reducing the number of infected cases, contagiousness and severity. Chlorpromazine (CPZ), an antipsychotic from the phenothiazine group, is known to inhibit clathrin-mediated endocytosis and has antiviral activity against severe acute respiratory syndrome coronavirus-1 (SARS-CoV-1) and Middle East respiratory syndrome coronavirus. The aim of this in-vitro study was to test CPZ against SARS-CoV-2 in monkey and human cells. MATERIALS AND METHODS: Monkey VeroE6 cells and human alveolar basal epithelial A549-ACE2 cells were infected with SARS-CoV-2 in the presence of various concentrations of CPZ. Supernatants were harvested at day 2 and analysed by quantitative reverse transcription polymerase chain reaction (RT-qPCR) for the presence of SARS-CoV-2 RNA. Cell viability was assessed in non-infected cells. RESULTS: CPZ was found to have antiviral activity against SARS-CoV-2 in monkey VeroE6 cells, with a half maximal inhibitory concentration (IC(50)) of 8.2 microM, half maximal cytotoxic concentration (CC(50)) of 13.5 microM, and selectivity index (SI) of 1.65. In human A549-ACE2 cells, CPZ was also found to have anti-SARS-CoV-2 activity, with IC(50) of 11.3 microM, CC(50) of 23.1 microM and SI of 2.04. DISCUSSION: Although the measured SI values are low, the IC(50) values measured in vitro may translate to CPZ dosages used in routine clinical practice because of the high biodistribution of CPZ in lungs and saliva. Also, the distribution of CPZ in brain could be of interest for treating or preventing neurological and psychiatric forms of COVID-19. CONCLUSIONS: These preclinical findings support clinical investigation of the repurposing of CPZ, a drug with mild side effects, in the treatment of patients with COVID-19. Twit Text FOAVip Inhibition of the replication of SARS-CoV-2 in human cells by the FDA-approved drug chlorpromazine ????Int J Antimicrob Agents http://www.kidney.de/pget.php?&tw=1&pmid=33387629 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33387629 #FOAvip English Wikipedia <ref>{{Cite pmid|33387629}}</ref> Inhibition of the replication of SARS-CoV-2 in human cells by the FDA-approved drug chlorpromazine #MMPMID33387629 Plaze M; Attali D; Prot M; Petit AC; Blatzer M; Vinckier F; Levillayer L; Chiaravalli J; Perin-Dureau F; Cachia A; Friedlander G; Chretien F; Simon-Loriere E; Gaillard R Int J Antimicrob Agents 2021[Mar]; 57 (3): 106274 PMID33387629show ga INTRODUCTION: Urgent action is needed to fight the ongoing coronavirus disease 2019 (COVID-19) pandemic by reducing the number of infected cases, contagiousness and severity. Chlorpromazine (CPZ), an antipsychotic from the phenothiazine group, is known to inhibit clathrin-mediated endocytosis and has antiviral activity against severe acute respiratory syndrome coronavirus-1 (SARS-CoV-1) and Middle East respiratory syndrome coronavirus. The aim of this in-vitro study was to test CPZ against SARS-CoV-2 in monkey and human cells. MATERIALS AND METHODS: Monkey VeroE6 cells and human alveolar basal epithelial A549-ACE2 cells were infected with SARS-CoV-2 in the presence of various concentrations of CPZ. Supernatants were harvested at day 2 and analysed by quantitative reverse transcription polymerase chain reaction (RT-qPCR) for the presence of SARS-CoV-2 RNA. Cell viability was assessed in non-infected cells. RESULTS: CPZ was found to have antiviral activity against SARS-CoV-2 in monkey VeroE6 cells, with a half maximal inhibitory concentration (IC(50)) of 8.2 microM, half maximal cytotoxic concentration (CC(50)) of 13.5 microM, and selectivity index (SI) of 1.65. In human A549-ACE2 cells, CPZ was also found to have anti-SARS-CoV-2 activity, with IC(50) of 11.3 microM, CC(50) of 23.1 microM and SI of 2.04. DISCUSSION: Although the measured SI values are low, the IC(50) values measured in vitro may translate to CPZ dosages used in routine clinical practice because of the high biodistribution of CPZ in lungs and saliva. Also, the distribution of CPZ in brain could be of interest for treating or preventing neurological and psychiatric forms of COVID-19. CONCLUSIONS: These preclinical findings support clinical investigation of the repurposing of CPZ, a drug with mild side effects, in the treatment of patients with COVID-19. |*Drug Repositioning[MESH] |A549 Cells[MESH] |Animals[MESH] |Antiviral Agents/*pharmacology[MESH] |COVID-19 Drug Treatment[MESH] |Cell Line[MESH] |Chlorocebus aethiops[MESH] |Chlorpromazine/pharmacokinetics/*pharmacology[MESH] |Humans[MESH] |SARS-CoV-2/*drug effects[MESH] |Tissue Distribution[MESH] |Vero Cells[MESH]Inhibition of the replication of SARS-CoV-2 in human cells by the FDA-(epmc).pdf DeepDyve Pubget Overpricing