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10.1371/journal.pone.0244824 http://scihub22266oqcxt.onion/10.1371/journal.pone.0244824 33382830!7774962!33382830     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=33382830&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       PLoS+One 2020 ; 15 (12): e0244824 Nephropedia Template TP {{tp|p=33382830|t=2020. A direct RT-qPCR approach to test large numbers of individuals for SARS-CoV-2 |pdf=|usr=}}{{33382830}} gab.com Text A direct RT-qPCR approach to test large numbers of individuals for SARS-CoV-2 JO: PLoS One http://www.kidney.de/pget.php?&tw=1&pmid=33382830 #FOAvip SARS-CoV-2 causes substantial morbidity and mortality in elderly and immunocompromised individuals, particularly in retirement homes, where transmission from asymptomatic staff and visitors may introduce the infection. Here we present a cheap and fast screening method based on direct RT-qPCR to detect SARS-CoV-2 in single or pooled gargle lavages ("mouthwashes"). This method detects individuals with large viral loads (Ct</=29) and we use it to test all staff at a nursing home daily over a period of three weeks in order to reduce the risk that the infection penetrates the facility. This or similar approaches can be implemented to protect hospitals, nursing homes and other institutions in this and future viral epidemics. Twit Text FOAVip A direct RT-qPCR approach to test large numbers of individuals for SARS-CoV-2 ????PLoS One http://www.kidney.de/pget.php?&tw=1&pmid=33382830 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33382830 #FOAvip English Wikipedia <ref>{{Cite pmid|33382830}}</ref> A direct RT-qPCR approach to test large numbers of individuals for SARS-CoV-2 #MMPMID33382830 Maricic T; Nickel O; Aximu-Petri A; Essel E; Gansauge M; Kanis P; Macak D; Richter J; Riesenberg S; Bokelmann L; Zeberg H; Meyer M; Borte S; Paabo S PLoS One 2020[]; 15 (12): e0244824 PMID33382830show ga SARS-CoV-2 causes substantial morbidity and mortality in elderly and immunocompromised individuals, particularly in retirement homes, where transmission from asymptomatic staff and visitors may introduce the infection. Here we present a cheap and fast screening method based on direct RT-qPCR to detect SARS-CoV-2 in single or pooled gargle lavages ("mouthwashes"). This method detects individuals with large viral loads (Ct |*COVID-19 Nucleic Acid Testing[MESH] |*COVID-19/diagnosis/genetics[MESH] |*Mass Screening[MESH] |*Real-Time Polymerase Chain Reaction[MESH] |Humans[MESH]A direct RT-qPCR approach to test large numbers of individuals for SAR(epmc).pdf DeepDyve Pubget Overpricing