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10.1038/s41598-020-78771-8 http://scihub22266oqcxt.onion/10.1038/s41598-020-78771-8 33380736!7773739!33380736     free     free     free Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Sci+Rep 2020 ; 10 (1): 22425 Nephropedia Template TP {{tp|p=33380736|t=2020. Hydrogel particles improve detection of SARS-CoV-2 RNA from multiple sample types |pdf=|usr=}}{{33380736}} gab.com Text Hydrogel particles improve detection of SARS-CoV-2 RNA from multiple sample types JO: Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=33380736 #FOAvip Here we present a rapid and versatile method for capturing and concentrating SARS-CoV-2 from contrived transport medium and saliva samples using affinity-capture magnetic hydrogel particles. We demonstrate that the method concentrates virus from 1 mL samples prior to RNA extraction, substantially improving detection of virus using real-time RT-PCR across a range of viral titers (100-1,000,000 viral copies/mL) and enabling detection of virus using the 2019 nCoV CDC EUA Kit down to 100 viral copies/mL. This method is compatible with commercially available nucleic acid extraction kits (i.e., from Qiagen) and a simple heat and detergent method that extracts viral RNA directly off the particle, allowing a sample processing time of 10 min. We furthermore tested our method in transport medium diagnostic remnant samples that previously had been tested for SARS-CoV-2, showing that our method not only correctly identified all positive samples but also substantially improved detection of the virus in low viral load samples. The average improvement in cycle threshold value across all viral titers tested was 3.1. Finally, we illustrate that our method could potentially be used to enable pooled testing, as we observed considerable improvement in the detection of SARS-CoV-2 RNA from sample volumes of up to 10 mL. Twit Text FOAVip Hydrogel particles improve detection of SARS-CoV-2 RNA from multiple sample types ????Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=33380736 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33380736 #FOAvip English Wikipedia <ref>{{Cite pmid|33380736}}</ref> Hydrogel particles improve detection of SARS-CoV-2 RNA from multiple sample types #MMPMID33380736 Barclay RA; Akhrymuk I; Patnaik A; Callahan V; Lehman C; Andersen P; Barbero R; Barksdale S; Dunlap R; Goldfarb D; Jones-Roe T; Kelly R; Kim B; Miao S; Munns A; Munns D; Patel S; Porter E; Ramsey R; Sahoo S; Swahn O; Warsh J; Kehn-Hall K; Lepene B Sci Rep 2020[Dec]; 10 (1): 22425 PMID33380736show ga Here we present a rapid and versatile method for capturing and concentrating SARS-CoV-2 from contrived transport medium and saliva samples using affinity-capture magnetic hydrogel particles. We demonstrate that the method concentrates virus from 1 mL samples prior to RNA extraction, substantially improving detection of virus using real-time RT-PCR across a range of viral titers (100-1,000,000 viral copies/mL) and enabling detection of virus using the 2019 nCoV CDC EUA Kit down to 100 viral copies/mL. This method is compatible with commercially available nucleic acid extraction kits (i.e., from Qiagen) and a simple heat and detergent method that extracts viral RNA directly off the particle, allowing a sample processing time of 10 min. We furthermore tested our method in transport medium diagnostic remnant samples that previously had been tested for SARS-CoV-2, showing that our method not only correctly identified all positive samples but also substantially improved detection of the virus in low viral load samples. The average improvement in cycle threshold value across all viral titers tested was 3.1. Finally, we illustrate that our method could potentially be used to enable pooled testing, as we observed considerable improvement in the detection of SARS-CoV-2 RNA from sample volumes of up to 10 mL. |COVID-19 Testing/*methods[MESH] |COVID-19/*diagnosis[MESH] |Diagnostic Tests, Routine[MESH] |Humans[MESH] |Hydrogels/*chemistry[MESH] |Nasopharynx/*virology[MESH] |RNA, Viral/*analysis[MESH] |Real-Time Polymerase Chain Reaction[MESH] |SARS-CoV-2/isolation & purification[MESH] |Saliva/*virology[MESH] |Sensitivity and Specificity[MESH] |Specimen Handling[MESH]Hydrogel particles improve detection of SARS-CoV-2 RNA from multiple s(epmc).pdf DeepDyve Pubget Overpricing