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10.1080/14737159.2021.1865807 http://scihub22266oqcxt.onion/10.1080/14737159.2021.1865807 33380245!7784781!33380245     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Expert+Rev+Mol+Diagn 2021 ; 21 (1): 119-129 Nephropedia Template TP {{tp|p=33380245|t=2021. Developing multiplex ddPCR assays for SARS-CoV-2 detection based on probe mix and amplitude based multiplexing |pdf=|usr=}}{{33380245}} gab.com Text Developing multiplex ddPCR assays for SARS-CoV-2 detection based on probe mix and amplitude based multiplexing JO: Expert Rev Mol Diagn http://www.kidney.de/pget.php?&tw=1&pmid=33380245 #FOAvip Introduction: With the ongoing SARS-CoV-2 pandemic, different articles have been published highlighting the superiority of droplet digital PCR (ddPCR) over the gold-standard reverse transcription PCR (RT-PCR) in SARS-CoV-2 detection. However, few studies have been reported on developing multiplex ddPCR assays for SARS-CoV-2 detection and their performance. This study shows steps on how to develop different ddPCR SAR-CoV-2 assays including higher order multiplex assays for SARS-CoV-2 detection and antiviral screening.Methods: Using multiple primer/probe sets, we developed, optimized, and analyzed the performance of simplex (1 target), duplex (2 targets), triplex probe mix (3 targets), and quadruplex (4 targets) SARS-CoV-2 ddPCR assays based on a two-color ddPCR detection system.Results: Results showed that the quadruplex assay had similar limits of detection and accuracy to the lower multiplex assays. Analyzing 94 clinical samples demonstrated that the ddPCR triplex probe mix assay had better sensitivity than the RT-qPCR assay. Additionally, the ddPCR multiplex assay showed that remdesivir could inhibit the growth of SARS-CoV-2 in vitro while another testing drug could not.Conclusion: Our research shows that developing multiplex ddPCR assays is possible by combing probe mix and amplitude-based multiplexing, which will help in developing multiplexed ddPCR assays for different SARS-CoV-2 applications. Twit Text FOAVip Developing multiplex ddPCR assays for SARS-CoV-2 detection based on probe mix and amplitude based multiplexing ????Expert Rev Mol Diagn http://www.kidney.de/pget.php?&tw=1&pmid=33380245 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33380245 #FOAvip English Wikipedia <ref>{{Cite pmid|33380245}}</ref> Developing multiplex ddPCR assays for SARS-CoV-2 detection based on probe mix and amplitude based multiplexing #MMPMID33380245 Nyaruaba R; Li C; Mwaliko C; Mwau M; Odiwuor N; Muturi E; Muema C; Xiong J; Li J; Yu J; Wei H Expert Rev Mol Diagn 2021[Jan]; 21 (1): 119-129 PMID33380245show ga Introduction: With the ongoing SARS-CoV-2 pandemic, different articles have been published highlighting the superiority of droplet digital PCR (ddPCR) over the gold-standard reverse transcription PCR (RT-PCR) in SARS-CoV-2 detection. However, few studies have been reported on developing multiplex ddPCR assays for SARS-CoV-2 detection and their performance. This study shows steps on how to develop different ddPCR SAR-CoV-2 assays including higher order multiplex assays for SARS-CoV-2 detection and antiviral screening.Methods: Using multiple primer/probe sets, we developed, optimized, and analyzed the performance of simplex (1 target), duplex (2 targets), triplex probe mix (3 targets), and quadruplex (4 targets) SARS-CoV-2 ddPCR assays based on a two-color ddPCR detection system.Results: Results showed that the quadruplex assay had similar limits of detection and accuracy to the lower multiplex assays. Analyzing 94 clinical samples demonstrated that the ddPCR triplex probe mix assay had better sensitivity than the RT-qPCR assay. Additionally, the ddPCR multiplex assay showed that remdesivir could inhibit the growth of SARS-CoV-2 in vitro while another testing drug could not.Conclusion: Our research shows that developing multiplex ddPCR assays is possible by combing probe mix and amplitude-based multiplexing, which will help in developing multiplexed ddPCR assays for different SARS-CoV-2 applications. |*COVID-19 Nucleic Acid Testing[MESH] |Antiviral Agents/pharmacology[MESH] |COVID-19/*diagnosis[MESH] |DNA Primers/genetics[MESH] |False Positive Reactions[MESH] |Humans[MESH] |Limit of Detection[MESH] |Multiplex Polymerase Chain Reaction/*methods[MESH] |Pandemics[MESH] |RNA, Viral/isolation & purification[MESH] |Real-Time Polymerase Chain Reaction/methods[MESH] |Reproducibility of Results[MESH] |SARS-CoV-2/*isolation & purification[MESH] |Sensitivity and Specificity[MESH] |Temperature[MESH]Developing multiplex ddPCR assays for SARS-CoV-2 detection based on pr(epmc).pdf DeepDyve Pubget Overpricing