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10.3390/vaccines9010013 http://scihub22266oqcxt.onion/10.3390/vaccines9010013 33379160!7824240!33379160     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=33379160&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Vaccines+(Basel) 2020 ; 9 (1): ä Nephropedia Template TP {{tp|p=33379160|t=2020. Comparison of Four SARS-CoV-2 Neutralization Assays |pdf=|usr=}}{{33379160}} gab.com Text Comparison of Four SARS-CoV-2 Neutralization Assays JO: Vaccines (Basel) http://www.kidney.de/pget.php?&tw=1&pmid=33379160 #FOAvip Neutralizing antibodies are a major correlate of protection for many viruses including the novel coronavirus SARS-CoV-2. Thus, vaccine candidates should potently induce neutralizing antibodies to render effective protection from infection. A variety of in vitro assays for the detection of SARS-CoV-2 neutralizing antibodies has been described. However, validation of the different assays against each other is important to allow comparison of different studies. Here, we compared four different SARS-CoV-2 neutralization assays using the same set of patient samples. Two assays used replication competent SARS-CoV-2, a focus forming assay and a TCID(50)-based assay, while the other two assays used replication defective lentiviral or vesicular stomatitis virus (VSV)-based particles pseudotyped with SARS-CoV-2 spike. All assays were robust and produced highly reproducible neutralization titers. Titers of neutralizing antibodies correlated well between the different assays and with the titers of SARS-CoV-2 S-protein binding antibodies detected in an ELISA. Our study showed that commonly used SARS-CoV-2 neutralization assays are robust and that results obtained with different assays are comparable. Twit Text FOAVip Comparison of Four SARS-CoV-2 Neutralization Assays ????Vaccines (Basel) http://www.kidney.de/pget.php?&tw=1&pmid=33379160 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33379160 #FOAvip English Wikipedia <ref>{{Cite pmid|33379160}}</ref> Comparison of Four SARS-CoV-2 Neutralization Assays #MMPMID33379160 Riepler L; Rossler A; Falch A; Volland A; Borena W; von Laer D; Kimpel J Vaccines (Basel) 2020[Dec]; 9 (1): ä PMID33379160show ga Neutralizing antibodies are a major correlate of protection for many viruses including the novel coronavirus SARS-CoV-2. Thus, vaccine candidates should potently induce neutralizing antibodies to render effective protection from infection. A variety of in vitro assays for the detection of SARS-CoV-2 neutralizing antibodies has been described. However, validation of the different assays against each other is important to allow comparison of different studies. Here, we compared four different SARS-CoV-2 neutralization assays using the same set of patient samples. Two assays used replication competent SARS-CoV-2, a focus forming assay and a TCID(50)-based assay, while the other two assays used replication defective lentiviral or vesicular stomatitis virus (VSV)-based particles pseudotyped with SARS-CoV-2 spike. All assays were robust and produced highly reproducible neutralization titers. Titers of neutralizing antibodies correlated well between the different assays and with the titers of SARS-CoV-2 S-protein binding antibodies detected in an ELISA. Our study showed that commonly used SARS-CoV-2 neutralization assays are robust and that results obtained with different assays are comparable. äComparison of Four SARS-CoV-2 Neutralization Assays (epmc).pdf DeepDyve Pubget Overpricing