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10.1016/j.talanta.2020.121850 http://scihub22266oqcxt.onion/10.1016/j.talanta.2020.121850 33379066!7657100!33379066     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Talanta 2021 ; 224 (ä): 121850 Nephropedia Template TP {{tp|p=33379066|t=2021. High-quality RT-PCR with chemically modified RNA controls |pdf=|usr=}}{{33379066}} gab.com Text High-quality RT-PCR with chemically modified RNA controls JO: Talanta http://www.kidney.de/pget.php?&tw=1&pmid=33379066 #FOAvip In detecting infectious diseases, such as coronavirus 2019 (COVID-19), real-time reverse-transcription polymerase chain reaction (RT-PCR) is one of the most important technologies for RNA detection and disease diagnosis. To achieve high quality assurance, appropriate positive and negative controls are critical for disease detection using RT-PCR kits. In this study, we have found that commercial kits often adopt DNAs instead of RNAs as the positive controls, which can't report the kit problems in reverse transcription, thereby increasing risk of the false negative results when testing patient samples. To face the challenge, we have proposed and developed the chemically modified RNAs, such as phosphoroselenaote and phosphorothioate RNAs (Se-RNA and S-RNA), as the controls. We have found that while demonstrating the high thermostability, biostability, chemostability and exclusivity (or specificity), both Se-RNA and S-RNA can be fine templates for reverse transcription, indicating their potentials as both positive and negative controls for RT-PCR kits. Twit Text FOAVip High-quality RT-PCR with chemically modified RNA controls ????Talanta http://www.kidney.de/pget.php?&tw=1&pmid=33379066 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33379066 #FOAvip English Wikipedia <ref>{{Cite pmid|33379066}}</ref> High-quality RT-PCR with chemically modified RNA controls #MMPMID33379066 Luo G; Zhang J; Zhang S; Hu B; Hu L; Huang Z Talanta 2021[Mar]; 224 (ä): 121850 PMID33379066show ga In detecting infectious diseases, such as coronavirus 2019 (COVID-19), real-time reverse-transcription polymerase chain reaction (RT-PCR) is one of the most important technologies for RNA detection and disease diagnosis. To achieve high quality assurance, appropriate positive and negative controls are critical for disease detection using RT-PCR kits. In this study, we have found that commercial kits often adopt DNAs instead of RNAs as the positive controls, which can't report the kit problems in reverse transcription, thereby increasing risk of the false negative results when testing patient samples. To face the challenge, we have proposed and developed the chemically modified RNAs, such as phosphoroselenaote and phosphorothioate RNAs (Se-RNA and S-RNA), as the controls. We have found that while demonstrating the high thermostability, biostability, chemostability and exclusivity (or specificity), both Se-RNA and S-RNA can be fine templates for reverse transcription, indicating their potentials as both positive and negative controls for RT-PCR kits. |COVID-19 Nucleic Acid Testing/instrumentation/*methods[MESH] |COVID-19/*diagnosis[MESH] |DNA, Viral/analysis[MESH] |False Negative Reactions[MESH] |Humans[MESH] |RNA Stability[MESH] |RNA, Viral/*analysis/chemistry[MESH] |Reagent Kits, Diagnostic[MESH] |Real-Time Polymerase Chain Reaction/instrumentation/*methods[MESH] |Reverse Transcriptase Polymerase Chain Reaction/instrumentation/*methods[MESH]High-quality RT-PCR with chemically modified RNA controls (epmc).pdf DeepDyve Pubget Overpricing