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Deprecated: Implicit conversion from float 332.4 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Bioessays 2021 ; 43 (3): e2000257 Nephropedia Template TP
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Microscopy-based assay for semi-quantitative detection of SARS-CoV-2 specific antibodies in human sera: A semi-quantitative, high throughput, microscopy-based assay expands existing approaches to measure SARS-CoV-2 specific antibody levels in human sera #MMPMID33377226
Pape C; Remme R; Wolny A; Olberg S; Wolf S; Cerrone L; Cortese M; Klaus S; Lucic B; Ullrich S; Anders-Osswein M; Wolf S; Cerikan B; Neufeldt CJ; Ganter M; Schnitzler P; Merle U; Lusic M; Boulant S; Stanifer M; Bartenschlager R; Hamprecht FA; Kreshuk A; Tischer C; Krausslich HG; Muller B; Laketa V
Bioessays 2021[Mar]; 43 (3): e2000257 PMID33377226show ga
Emergence of the novel pathogenic coronavirus SARS-CoV-2 and its rapid pandemic spread presents challenges that demand immediate attention. Here, we describe the development of a semi-quantitative high-content microscopy-based assay for detection of three major classes (IgG, IgA, and IgM) of SARS-CoV-2 specific antibodies in human samples. The possibility to detect antibodies against the entire viral proteome together with a robust semi-automated image analysis workflow resulted in specific, sensitive and unbiased assay that complements the portfolio of SARS-CoV-2 serological assays. Sensitive, specific and quantitative serological assays are urgently needed for a better understanding of humoral immune response against the virus as a basis for developing public health strategies to control viral spread. The procedure described here has been used for clinical studies and provides a general framework for the application of quantitative high-throughput microscopy to rapidly develop serological assays for emerging virus infections.