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10.1002/jmv.26761 http://scihub22266oqcxt.onion/10.1002/jmv.26761 33368332!ä!33368332 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Med+Virol 2021 ; 93 (4): 2439-2445 Nephropedia Template TP {{tp|p=33368332|t=2021. Quantitative detection of SARS-CoV-2 RNA in nasopharyngeal samples from infected patients with mild disease |pdf=|usr=}}{{33368332}} gab.com Text Quantitative detection of SARS-CoV-2 RNA in nasopharyngeal samples from infected patients with mild disease JO: J Med Virol http://www.kidney.de/pget.php?&tw=1&pmid=33368332 #FOAvip Diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS-COV-2) cases is based on the count of real-time reverse transcription-plymerase chain reaction (RT-PCR) positive people. Viral load by real-time RT-PCR has been suggested as a biomarker of the SARS-CoV-2 infection. However, the association of viral load and severity of the disease is not yet resolved. Nasopharyngeal samples from 458 patients were tested by RT-PCR for SARS-CoV-2 diagnosis. Relative quantitation was made by the comparative threshold cycle (DeltaDeltaC(t) ) formula between ORF1ab viral and RNase P housekeeping genes. Absolute viral load was calculate using a reference positive control. Most prevalent clinical signs were cough (75.8%), myalgia (66.7%), and fever (48.5%). Hypertension (18.2%), neurological diseases (15.1%), and asthma and hypothyroidism (12.1%) were most frequent comorbidities. Fever, either as an exclusive symptom or combined with others, was associated with high viral loads ( 2-??Ct range, 35.65-155.16; 4.25-4.89 log(10) RNA copies/test]). During the first week after onset of symptoms in mild patients up to 60 years-old was detected the peak of viral load. Children under 10 years old have a high viral load (313.84; 2.50) in the first 2 days postinfection with a sharp decline thereafter. Cases between 10 and 49 years old mostly showed low and moderate viral load during the first 2 days postinfection (range, 0.03 to 17.24; -1.50 to 1.24). Patients over 60 years old have high viral load up to the second week after the onset of symptoms (range, 25.32-155.42; 1.40-2.19), indicating the longer presence of the virus in them. These findings suggest the viral load in nasopharyngeal swabs would help to monitor the SARS-CoV-2 infection in mild coronavirus disease 2019 cases. Twit Text FOAVip Quantitative detection of SARS-CoV-2 RNA in nasopharyngeal samples from infected patients with mild disease ????J Med Virol http://www.kidney.de/pget.php?&tw=1&pmid=33368332 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33368332 #FOAvip English Wikipedia <ref>{{Cite pmid|33368332}}</ref> Quantitative detection of SARS-CoV-2 RNA in nasopharyngeal samples from infected patients with mild disease #MMPMID33368332 Bustos P; Tambley C; Acevedo A; Andrade W; Leal G; Vidal D; Roldan F; Fasce R; Ramirez E J Med Virol 2021[Apr]; 93 (4): 2439-2445 PMID33368332show ga Diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS-COV-2) cases is based on the count of real-time reverse transcription-plymerase chain reaction (RT-PCR) positive people. Viral load by real-time RT-PCR has been suggested as a biomarker of the SARS-CoV-2 infection. However, the association of viral load and severity of the disease is not yet resolved. Nasopharyngeal samples from 458 patients were tested by RT-PCR for SARS-CoV-2 diagnosis. Relative quantitation was made by the comparative threshold cycle (DeltaDeltaC(t) ) formula between ORF1ab viral and RNase P housekeeping genes. Absolute viral load was calculate using a reference positive control. Most prevalent clinical signs were cough (75.8%), myalgia (66.7%), and fever (48.5%). Hypertension (18.2%), neurological diseases (15.1%), and asthma and hypothyroidism (12.1%) were most frequent comorbidities. Fever, either as an exclusive symptom or combined with others, was associated with high viral loads ( 2-??Ct range, 35.65-155.16; 4.25-4.89 log(10) RNA copies/test]). During the first week after onset of symptoms in mild patients up to 60 years-old was detected the peak of viral load. Children under 10 years old have a high viral load (313.84; 2.50) in the first 2 days postinfection with a sharp decline thereafter. Cases between 10 and 49 years old mostly showed low and moderate viral load during the first 2 days postinfection (range, 0.03 to 17.24; -1.50 to 1.24). Patients over 60 years old have high viral load up to the second week after the onset of symptoms (range, 25.32-155.42; 1.40-2.19), indicating the longer presence of the virus in them. These findings suggest the viral load in nasopharyngeal swabs would help to monitor the SARS-CoV-2 infection in mild coronavirus disease 2019 cases. |Adolescent[MESH] |Adult[MESH] |Aged[MESH] |Aged, 80 and over[MESH] |COVID-19 Testing/*methods[MESH] |COVID-19/diagnosis/*virology[MESH] |Child[MESH] |Child, Preschool[MESH] |Diagnostic Tests, Routine[MESH] |Female[MESH] |Humans[MESH] |Infant[MESH] |Male[MESH] |Middle Aged[MESH] |Nasopharynx/*virology[MESH] |Polyproteins/genetics[MESH] |RNA, Viral/*isolation & purification[MESH] |Real-Time Polymerase Chain Reaction/methods[MESH] |Ribonuclease P/genetics[MESH] |SARS-CoV-2/*genetics/isolation & purification[MESH] |Viral Load[MESH] |Viral Proteins/genetics[MESH]Quantitative detection of SARS-CoV-2 RNA in nasopharyngeal samples fro(epmc).pdf DeepDyve Pubget Overpricing