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10.1016/j.bpj.2020.11.2277

http://scihub22266oqcxt.onion/10.1016/j.bpj.2020.11.2277
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suck abstract from ncbi


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pmid33359834      Biophys+J 2021 ; 120 (3): 504-516
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  • The Molecular Mechanism of Domain Swapping of the C-Terminal Domain of the SARS-Coronavirus Main Protease #MMPMID33359834
  • Terse VL; Gosavi S
  • Biophys J 2021[Feb]; 120 (3): 504-516 PMID33359834show ga
  • In three-dimensional domain swapping, two protein monomers exchange a part of their structures to form an intertwined homodimer, whose subunits resemble the monomer. Several viral proteins domain swap to increase their structural complexity or functional avidity. The main protease (M(pro)) of the severe acute respiratory syndrome (SARS) coronavirus proteolyzes viral polyproteins and has been a target for anti-SARS drug design. Domain swapping in the alpha-helical C-terminal domain of M(pro) (M(pro)C) locks M(pro) into a hyperactive octameric form that is hypothesized to promote the early stages of viral replication. However, in the absence of a complete molecular understanding of the mechanism of domain swapping, investigations into the biological relevance of this octameric M(pro) have stalled. Isolated M(pro)C can exist as a monomer or a domain-swapped dimer. Here, we investigate the mechanism of domain swapping of M(pro)C using coarse-grained structure-based models and molecular dynamics simulations. Our simulations recapitulate several experimental features of M(pro)C folding. Further, we find that a contact between a tryptophan in the M(pro)C domain-swapping hinge and an arginine elsewhere forms early during folding, modulates the folding route, and promotes domain swapping to the native structure. An examination of the sequence and the structure of the tryptophan containing hinge loop shows that it has a propensity to form multiple secondary structures and contacts, indicating that it could be stabilized into either the monomer- or dimer-promoting conformations by mutations or ligand binding. Finally, because all residues in the tryptophan loop are identical in SARS-CoV and SARS-CoV-2, mutations that modulate domain swapping may provide insights into the role of octameric M(pro) in the early-stage viral replication of both viruses.
  • |*Models, Molecular[MESH]
  • |Peptide Hydrolases/*chemistry/*metabolism[MESH]
  • |Protein Domains[MESH]
  • |Protein Folding[MESH]


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