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10.1016/j.jviromet.2020.114056

http://scihub22266oqcxt.onion/10.1016/j.jviromet.2020.114056
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33359615!ä!33359615

suck abstract from ncbi


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pmid33359615      J+Virol+Methods 2021 ; 289 (ä): 114056
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  • Application of real-time PCR to significantly reduce the time to obtain recombinant MVA virus #MMPMID33359615
  • Orlova OV; Glazkova DV; Tsyganova GM; Antoshkina IV; Mintaev RR; Tikhonov AS; Bogoslovskaya EV; Shipulin GA
  • J Virol Methods 2021[Mar]; 289 (ä): 114056 PMID33359615show ga
  • Obtaining a pure recombinant Modified Vaccinia Ankara (MVA) virus is a multistage, time-consuming procedure. We describe a novel single-tube real-time PCR which enables determination of the amount of wild type and recombinant viruses and their ratio in plaques. Use of the real-time PCR significantly reduce the time and efforts needed to obtain purified recombinant MVA. The new approach has been applied to generate recombinant MVAs encoding different SARS-COV-2 antigens.
  • |*Antigens, Viral[MESH]
  • |*Genetic Vectors[MESH]
  • |Animals[MESH]
  • |Cell Line[MESH]
  • |Humans[MESH]
  • |Real-Time Polymerase Chain Reaction[MESH]
  • |SARS-CoV-2/*genetics[MESH]


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