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10.1016/j.jim.2020.112952 http://scihub22266oqcxt.onion/10.1016/j.jim.2020.112952 33358997!7867647!33358997     free     free     free Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Immunol+Methods 2021 ; 490 (ä): 112952 Nephropedia Template TP {{tp|p=33358997|t=2021. Multiplex bead binding assays using off-the-shelf components and common flow cytometers |pdf=|usr=}}{{33358997}} gab.com Text Multiplex bead binding assays using off-the-shelf components and common flow cytometers JO: J Immunol Methods http://www.kidney.de/pget.php?&tw=1&pmid=33358997 #FOAvip The ability to quantify protein-ligand interactions in an accurate and high-throughput manner is important in diverse areas of biology and medicine. Multiplex bead binding assays (MBBAs) are powerful methods that allow for simultaneous analysis of many protein-ligand interactions. Although there are a number of well-established MBBA platforms, there are few platforms suitable for research and development that offer rapid experimentation at low costs and without the need for specialized reagents or instruments dedicated for MBBA. Here, we describe a MBBA method that uses low-cost reagents and standard cytometers. The key innovation is the use of the essentially irreversible biotin-streptavidin interaction. We prepared a biotin-conjugated fluorescent dye and used it to produce streptavidin-coated magnetic beads that are labeled at distinct levels of fluorescence. We show the utility of our method in characterization of phage-displayed antibodies against multiple antigens of SARS-CoV-2, which substantially improves the throughput and dramatically reduces antigen consumption compared with conventional phage ELISA methods. This approach will make MBBAs more broadly accessible. Twit Text FOAVip Multiplex bead binding assays using off-the-shelf components and common flow cytometers ????J Immunol Methods http://www.kidney.de/pget.php?&tw=1&pmid=33358997 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33358997 #FOAvip English Wikipedia <ref>{{Cite pmid|33358997}}</ref> Multiplex bead binding assays using off-the-shelf components and common flow cytometers #MMPMID33358997 Hattori T; Koide A; Panchenko T; Romero LA; Teng KW; Corrado AD; Koide S J Immunol Methods 2021[Mar]; 490 (ä): 112952 PMID33358997show ga The ability to quantify protein-ligand interactions in an accurate and high-throughput manner is important in diverse areas of biology and medicine. Multiplex bead binding assays (MBBAs) are powerful methods that allow for simultaneous analysis of many protein-ligand interactions. Although there are a number of well-established MBBA platforms, there are few platforms suitable for research and development that offer rapid experimentation at low costs and without the need for specialized reagents or instruments dedicated for MBBA. Here, we describe a MBBA method that uses low-cost reagents and standard cytometers. The key innovation is the use of the essentially irreversible biotin-streptavidin interaction. We prepared a biotin-conjugated fluorescent dye and used it to produce streptavidin-coated magnetic beads that are labeled at distinct levels of fluorescence. We show the utility of our method in characterization of phage-displayed antibodies against multiple antigens of SARS-CoV-2, which substantially improves the throughput and dramatically reduces antigen consumption compared with conventional phage ELISA methods. This approach will make MBBAs more broadly accessible. |Bacterial Proteins/metabolism[MESH] |Biotin/analogs & derivatives/metabolism[MESH] |COVID-19 Serological Testing/*methods[MESH] |COVID-19/*diagnosis[MESH] |Cell Surface Display Techniques[MESH] |Flow Cytometry[MESH] |Fluorescent Dyes[MESH] |HEK293 Cells[MESH] |High-Throughput Screening Assays[MESH] |Humans[MESH] |Immunomagnetic Separation[MESH] |Microspheres[MESH] |Mutation/genetics[MESH] |Protein Binding[MESH] |SARS-CoV-2/genetics/*metabolism[MESH]Multiplex bead binding assays using off-the-shelf components and commo(epmc).pdf DeepDyve Pubget Overpricing