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10.1039/d0nr06893d http://scihub22266oqcxt.onion/10.1039/d0nr06893d 33351018!ä!33351018 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Nanoscale 2021 ; 13 (1): 388-396 Nephropedia Template TP {{tp|p=33351018|t=2021. Self-assembled chromogen-loaded polymeric cocoon for respiratory virus detection |pdf=|usr=}}{{33351018}} gab.com Text Self-assembled chromogen-loaded polymeric cocoon for respiratory virus detection JO: Nanoscale http://www.kidney.de/pget.php?&tw=1&pmid=33351018 #FOAvip Inspired by the self-assembly approach, in this work, the chromogen, 3,3',5,5'-tetramethylbenzidine (TMB), was successfully co-precipitated in aqueous solution to form collective nanoparticles (NPs) of signal molecules (TMB-NPs). Utilizing poly(lactide-co-glycolide) (PLGA) in the molecular delivery approach, the formed emulsion nanovesicle (TMB-NPs@PLGA) exhibits an enrichment of the collective signal molecules in a single antibody-antigen conjugation. A specific antibody-conjugated TMB-NPs@PLGA forms an immunocomplex sandwich structure upon the addition of influenza virus (IV)/A. The addition of dimethyl sulfoxide (DMSO) dissolves the PLGA nanovesicles, releasing the encapsulated TMB-NPs. Sequentially, the TMB-NPs release TMB molecules upon the addition of DMSO. The released TMB is catalytically oxidized by H(2)O(2) with self-assembled protein-inorganic nanoflowers, where copper nanoflowers (CuNFs) acted as the nanozyme. The developed immunoassay demonstrates high sensitivity for IV/A with a limit of detection (LOD) as low as 32.37 fg mL(-1) and 54.97 fg mL(-1) in buffer and serum, respectively. For practical needs, a clinically isolated IV/A/H3N2 and spike protein of SARS-CoV-2 were detected with the LODs of 17 pfu mL(-1) and 143 fg mL(-1), respectively. These results show the applicability of the advanced TMB-NPs@PLGA-based colorimetric sensor for the highly sensitive detection of airborne respiratory viruses. Twit Text FOAVip Self-assembled chromogen-loaded polymeric cocoon for respiratory virus detection ????Nanoscale http://www.kidney.de/pget.php?&tw=1&pmid=33351018 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33351018 #FOAvip English Wikipedia <ref>{{Cite pmid|33351018}}</ref> Self-assembled chromogen-loaded polymeric cocoon for respiratory virus detection #MMPMID33351018 Khoris IM; Ganganboina AB; Suzuki T; Park EY Nanoscale 2021[Jan]; 13 (1): 388-396 PMID33351018show ga Inspired by the self-assembly approach, in this work, the chromogen, 3,3',5,5'-tetramethylbenzidine (TMB), was successfully co-precipitated in aqueous solution to form collective nanoparticles (NPs) of signal molecules (TMB-NPs). Utilizing poly(lactide-co-glycolide) (PLGA) in the molecular delivery approach, the formed emulsion nanovesicle (TMB-NPs@PLGA) exhibits an enrichment of the collective signal molecules in a single antibody-antigen conjugation. A specific antibody-conjugated TMB-NPs@PLGA forms an immunocomplex sandwich structure upon the addition of influenza virus (IV)/A. The addition of dimethyl sulfoxide (DMSO) dissolves the PLGA nanovesicles, releasing the encapsulated TMB-NPs. Sequentially, the TMB-NPs release TMB molecules upon the addition of DMSO. The released TMB is catalytically oxidized by H(2)O(2) with self-assembled protein-inorganic nanoflowers, where copper nanoflowers (CuNFs) acted as the nanozyme. The developed immunoassay demonstrates high sensitivity for IV/A with a limit of detection (LOD) as low as 32.37 fg mL(-1) and 54.97 fg mL(-1) in buffer and serum, respectively. For practical needs, a clinically isolated IV/A/H3N2 and spike protein of SARS-CoV-2 were detected with the LODs of 17 pfu mL(-1) and 143 fg mL(-1), respectively. These results show the applicability of the advanced TMB-NPs@PLGA-based colorimetric sensor for the highly sensitive detection of airborne respiratory viruses. |*Respiratory Tract Infections/diagnosis/virology[MESH] |Benzidines/chemistry[MESH] |Biosensing Techniques/*methods[MESH] |COVID-19/diagnosis/virology[MESH] |Chromogenic Compounds/*chemistry[MESH] |Humans[MESH] |Hydrogen Peroxide[MESH] |Immunoassay/methods[MESH] |Influenza A Virus, H3N2 Subtype/*isolation & purification[MESH] |Influenza, Human/diagnosis/virology[MESH] |Limit of Detection[MESH] |Nanoparticles/chemistry[MESH] |Polylactic Acid-Polyglycolic Acid Copolymer/chemistry[MESH] |SARS-CoV-2/*isolation & purification[MESH]Self-assembled chromogen-loaded polymeric cocoon for respiratory virus(epmc).pdf DeepDyve Pubget Overpricing