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10.1177/1040638720974114 http://scihub22266oqcxt.onion/10.1177/1040638720974114 33350347!7758680!33350347     free     free     free Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Vet+Diagn+Invest 2021 ; 33 (1): 104-107 Nephropedia Template TP {{tp|p=33350347|t=2021. Development of a nanoparticle-assisted PCR assay to distinguish canine coronaviruses I and II |pdf=|usr=}}{{33350347}} gab.com Text Development of a nanoparticle-assisted PCR assay to distinguish canine coronaviruses I and II JO: J Vet Diagn Invest http://www.kidney.de/pget.php?&tw=1&pmid=33350347 #FOAvip Nanoparticle-assisted PCR (nanoPCR) is a novel method for the simple, rapid, and specific detection of viruses. We developed a nanoPCR method to detect and differentiate canine coronavirus I (CCoV I) and II (CCoV II). Primer pairs were designed against the M gene conserved region of CCoV I and CCoV II, producing specific fragments of 239 bp (CCoV I) and 105 bp (CCoV II). We optimized the annealing temperature and primer concentrations for the CCoV nanoPCR assay and assessed its sensitivity and specificity. Under optimized nanoPCR reaction conditions, the detection limits were 6.47 x 10(1) copies/muL for CCoV I and 6.91 x 10(2) copies/muL for CCoV II. No fragments were amplified using other canine viruses as templates. The sensitivity of the nanoPCR assay was 100-fold higher than that of a conventional RT-PCR assay. Among 60 clinical samples collected from Beijing, China, the assay detected 12% positive for CCoV I and 48% positive for CCoV II. Our nanoPCR method is an effective method to rapidly detect CCoV I and CCoV II alone, or as a mixed infection, in dogs. Twit Text FOAVip Development of a nanoparticle-assisted PCR assay to distinguish canine coronaviruses I and II ????J Vet Diagn Invest http://www.kidney.de/pget.php?&tw=1&pmid=33350347 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33350347 #FOAvip English Wikipedia <ref>{{Cite pmid|33350347}}</ref> Development of a nanoparticle-assisted PCR assay to distinguish canine coronaviruses I and II #MMPMID33350347 Qin T; Wang J; Cui SJ J Vet Diagn Invest 2021[Jan]; 33 (1): 104-107 PMID33350347show ga Nanoparticle-assisted PCR (nanoPCR) is a novel method for the simple, rapid, and specific detection of viruses. We developed a nanoPCR method to detect and differentiate canine coronavirus I (CCoV I) and II (CCoV II). Primer pairs were designed against the M gene conserved region of CCoV I and CCoV II, producing specific fragments of 239 bp (CCoV I) and 105 bp (CCoV II). We optimized the annealing temperature and primer concentrations for the CCoV nanoPCR assay and assessed its sensitivity and specificity. Under optimized nanoPCR reaction conditions, the detection limits were 6.47 x 10(1) copies/muL for CCoV I and 6.91 x 10(2) copies/muL for CCoV II. No fragments were amplified using other canine viruses as templates. The sensitivity of the nanoPCR assay was 100-fold higher than that of a conventional RT-PCR assay. Among 60 clinical samples collected from Beijing, China, the assay detected 12% positive for CCoV I and 48% positive for CCoV II. Our nanoPCR method is an effective method to rapidly detect CCoV I and CCoV II alone, or as a mixed infection, in dogs. |*Nanoparticles[MESH] |Animals[MESH] |Coinfection/veterinary/virology[MESH] |Coronavirus Infections/diagnosis/*veterinary/virology[MESH] |Coronavirus, Canine/classification/*genetics[MESH] |Dog Diseases/diagnosis/*virology[MESH] |Dogs[MESH]Development of a nanoparticle-assisted PCR assay to distinguish canine(epmc).pdf DeepDyve Pubget Overpricing