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10.1016/j.diagmicrobio.2020.115260

http://scihub22266oqcxt.onion/10.1016/j.diagmicrobio.2020.115260
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33340934!7654322!33340934
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suck abstract from ncbi


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pmid33340934      Diagn+Microbiol+Infect+Dis 2021 ; 99 (3): 115260
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  • Evaluation of the BioFire(R) COVID-19 test and Respiratory Panel 2 1 for rapid identification of SARS-CoV-2 in nasopharyngeal swab samples #MMPMID33340934
  • Eckbo EJ; Locher K; Caza M; Li L; Lavergne V; Charles M
  • Diagn Microbiol Infect Dis 2021[Mar]; 99 (3): 115260 PMID33340934show ga
  • The BioFire(R) COVID-19 Test and Respiratory Panel 2.1 (RP2.1) are rapid, fully automated assays for the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in nasopharyngeal swabs. In the case of the RP2.1, an additional 21 viral and bacterial pathogens can be detected. Both tests have received emergency use authorization from the U.S. Food & Drug Administration and Interim Order authorization from Health Canada for use in clinical laboratories. We evaluated the performance characteristics of these tests in comparison to a laboratory-developed real-time PCR assay targeting the viral RNA-dependent RNA polymerase and E genes. A total of 78 tests were performed using the BioFire COVID-19 Test, including 30 clinical specimens and 48 tests in a limit of detection study; 57 tests were performed using the RP2.1 for evaluation of SARS-CoV-2 detection, including 30 clinical specimens and 27 tests for limit of detection. Results showed 100% concordance between the BioFire assays and the laboratory-developed test for all clinical samples tested, and acceptable performance of both BioFire assays at their stated limits of detection. Conclusively, the BioFire COVID-19 Test and RP2.1 are highly sensitive assays that can be effectively used in the clinical laboratory for rapid SARS-CoV-2 testing.
  • |COVID-19 Testing/*methods/standards[MESH]
  • |COVID-19/*diagnosis[MESH]
  • |Clinical Laboratory Techniques/methods[MESH]
  • |Diagnostic Tests, Routine[MESH]
  • |Humans[MESH]
  • |Limit of Detection[MESH]
  • |Multiplex Polymerase Chain Reaction[MESH]
  • |Nasopharynx/*virology[MESH]
  • |Real-Time Polymerase Chain Reaction/methods[MESH]
  • |Reproducibility of Results[MESH]
  • |SARS-CoV-2/*isolation & purification[MESH]


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