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10.1016/j.ijid.2020.12.027

http://scihub22266oqcxt.onion/10.1016/j.ijid.2020.12.027
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suck abstract from ncbi


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pmid33326873      Int+J+Infect+Dis 2021 ; 103 (ä): 517-524
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  • Simultaneous detection and differentiation of SARS-CoV-2, influenza A virus and influenza B virus by one-step quadruplex real-time RT-PCR in patients with clinical manifestations #MMPMID33326873
  • Ni M; Xu H; Luo J; Liu W; Zhou D
  • Int J Infect Dis 2021[Feb]; 103 (ä): 517-524 PMID33326873show ga
  • OBJECTIVE: To develop a novel quadruplex real-time reverse transcription polymerase chain reaction (rRT-PCR) assay for the diagnosis of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infection, differential diagnosis and detection of co-infections. METHODS: A one-step quadruplex rRT-PCR assay was developed for simultaneous detection and differentiation of SARS-CoV-2 ORF1ab and N genes, influenza A virus (hIAV) and influenza B virus (hIBV). RESULTS: The quadruplex rRT-PCR assay had good sensitivity and specificity. Correlation coefficients and amplification efficiencies of all singleplex and quadruplex rRT-PCR reactions were within acceptable ranges. The 95% lower limits of detection for plasmid standards and positive nucleic acid extracts of the quadruplex rRT-PCR assay were 57.38-95.11 copies/muL and 114.65-154.25 copies/muL, respectively. Excellent results were attained for inter- and intra-assay reproducibility. Among these clinical samples, only four samples showed results inconsistent with the singleplex rRT-PCR assays. CONCLUSIONS: To the authors' knowledge, this is the first study to report a quadruplex rRT-PCR assay for the detection of two SARS-CoV-2 genes, hIAV and hIBV with perfect clinical performance.
  • |COVID-19/*diagnosis[MESH]
  • |Humans[MESH]
  • |Influenza A virus/*isolation & purification[MESH]
  • |Influenza B virus/*isolation & purification[MESH]
  • |Real-Time Polymerase Chain Reaction/*methods[MESH]
  • |Reverse Transcriptase Polymerase Chain Reaction/*methods[MESH]
  • |SARS-CoV-2/*isolation & purification[MESH]


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