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10.1177/1535370220975351 http://scihub22266oqcxt.onion/10.1177/1535370220975351 33325750!7746953!33325750     free     free     free Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Exp+Biol+Med+(Maywood) 2021 ; 246 (8): 960-970 Nephropedia Template TP {{tp|p=33325750|t=2021. Genomic analysis of SARS-CoV-2 reveals local viral evolution in Ghana |pdf=|usr=}}{{33325750}} gab.com Text Genomic analysis of SARS-CoV-2 reveals local viral evolution in Ghana JO: Exp Biol Med (Maywood) http://www.kidney.de/pget.php?&tw=1&pmid=33325750 #FOAvip The confirmed case fatality rate for the coronavirus disease 2019 (COVID-19) in Ghana has dropped from a peak of 2% in March to be consistently below 1% since May 2020. Globally, case fatality rates have been linked to the strains/clades of circulating severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) within a specific country. Here we present 46 whole genomes of SARS-CoV-2 circulating in Ghana, from two separate sequencing batches: 15 isolates from the early epidemic (March 12-April 1 2020) and 31 from later time-points ( 25-27 May 2020). Sequencing was carried out on an Illumina MiSeq system following an amplicon-based enrichment for SARS-CoV-2 cDNA. After genome assembly and quality control processes, phylogenetic analysis showed that the first batch of 15 genomes clustered into five clades: 19A, 19B, 20A, 20B, and 20C, whereas the second batch of 31 genomes clustered to only three clades 19B, 20A, and 20B. The imported cases (6/46) mapped to circulating viruses in their countries of origin, namely, India, Hungary, Norway, the United Kingdom, and the United States of America. All genomes mapped to the original Wuhan strain with high similarity (99.5-99.8%). All imported strains mapped to the European superclade A, whereas 5/9 locally infected individuals harbored the B4 clade, from the East Asian superclade B. Ghana appears to have 19B and 20B as the two largest circulating clades based on our sequence analyses. In line with global reports, the D614G linked viruses seem to be predominating. Comparison of Ghanaian SARS-CoV-2 genomes with global genomes indicates that Ghanaian strains have not diverged significantly from circulating strains commonly imported into Africa. The low level of diversity in our genomes may indicate lower levels of transmission, even for D614G viruses, which is consistent with the relatively low levels of infection reported in Ghana. Twit Text FOAVip Genomic analysis of SARS-CoV-2 reveals local viral evolution in Ghana ????Exp Biol Med (Maywood) http://www.kidney.de/pget.php?&tw=1&pmid=33325750 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33325750 #FOAvip English Wikipedia <ref>{{Cite pmid|33325750}}</ref> Genomic analysis of SARS-CoV-2 reveals local viral evolution in Ghana #MMPMID33325750 Ngoi JM; Quashie PK; Morang'a CM; Bonney JH; Amuzu DS; Kumordjie S; Asante IA; Bonney EY; Eshun M; Boatemaa L; Magnusen V; Kotey EN; Ndam NT; Tei-Maya F; Arjarquah AK; Obodai E; Otchere ID; Bediako Y; Mutungi JK; Amenga-Etego LN; Odoom JK; Anang AK; Kyei GB; Adu B; Ampofo WK; Awandare GA Exp Biol Med (Maywood) 2021[Apr]; 246 (8): 960-970 PMID33325750show ga The confirmed case fatality rate for the coronavirus disease 2019 (COVID-19) in Ghana has dropped from a peak of 2% in March to be consistently below 1% since May 2020. Globally, case fatality rates have been linked to the strains/clades of circulating severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) within a specific country. Here we present 46 whole genomes of SARS-CoV-2 circulating in Ghana, from two separate sequencing batches: 15 isolates from the early epidemic (March 12-April 1 2020) and 31 from later time-points ( 25-27 May 2020). Sequencing was carried out on an Illumina MiSeq system following an amplicon-based enrichment for SARS-CoV-2 cDNA. After genome assembly and quality control processes, phylogenetic analysis showed that the first batch of 15 genomes clustered into five clades: 19A, 19B, 20A, 20B, and 20C, whereas the second batch of 31 genomes clustered to only three clades 19B, 20A, and 20B. The imported cases (6/46) mapped to circulating viruses in their countries of origin, namely, India, Hungary, Norway, the United Kingdom, and the United States of America. All genomes mapped to the original Wuhan strain with high similarity (99.5-99.8%). All imported strains mapped to the European superclade A, whereas 5/9 locally infected individuals harbored the B4 clade, from the East Asian superclade B. Ghana appears to have 19B and 20B as the two largest circulating clades based on our sequence analyses. In line with global reports, the D614G linked viruses seem to be predominating. Comparison of Ghanaian SARS-CoV-2 genomes with global genomes indicates that Ghanaian strains have not diverged significantly from circulating strains commonly imported into Africa. The low level of diversity in our genomes may indicate lower levels of transmission, even for D614G viruses, which is consistent with the relatively low levels of infection reported in Ghana. |*Evolution, Molecular[MESH] |*Genome, Viral[MESH] |*Phylogeny[MESH] |COVID-19/epidemiology[MESH] |Ghana/epidemiology[MESH] |Humans[MESH]Genomic analysis of SARS-CoV-2 reveals local viral evolution in Ghana (epmc).pdf DeepDyve Pubget Overpricing