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10.1021/acs.analchem.0c03347

http://scihub22266oqcxt.onion/10.1021/acs.analchem.0c03347
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33319993!ä!33319993

suck abstract from ncbi


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pmid33319993      Anal+Chem 2021 ; 93 (2): 828-833
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  • Ultrafast Surface Plasmon Resonance Imaging Sensor via the High-Precision Four-Parameter-Based Spectral Curve Readjusting Method #MMPMID33319993
  • Wang X; Zeng Y; Zhou J; Chen J; Miyan R; Zhang H; Qu J; Ho HP; Gao BZ; Shao Y
  • Anal Chem 2021[Jan]; 93 (2): 828-833 PMID33319993show ga
  • A variety of surface plasmon resonance (SPR) sensing devices have been extensively used in biochemical detection for their characteristics of label-free, highly sensitive, and faster detecting. Among them, the spectrum-based SPR sensing devices have offered us great advantages in high-throughput sensing due to their large dynamic range and the possibility of detection resolution similar to that offered by angle interrogation. This paper demonstrates a spectrum-based SPR imaging sensing system with fast wavelength scanning capability achieved by an acousto-optic tunable filter (AOTF) and a low-cost and speckle-free halogen lamp implemented as the SPR excitation source. Especially, we developed a novel four-parameter-based spectral curve readjusting (4-PSCR) method for data processing, which offered us a faster and more accurate spectral data curve fitting process than the traditional polynomial fitting method. With the configuration, we have also conducted an SPR high-throughput detection of the novel coronavirus (COVID-19) spike protein, proving its application possibility in the screening of COVID-19 with high accuracy. We believe that the higher sensitivity and accuracy of the system have made it readily used in biochemical imaging and detecting applications.
  • |Algorithms[MESH]
  • |COVID-19/diagnosis/virology[MESH]
  • |Humans[MESH]
  • |Limit of Detection[MESH]
  • |Optics and Photonics[MESH]
  • |SARS-CoV-2/isolation & purification/metabolism[MESH]
  • |Spike Glycoprotein, Coronavirus/*analysis/metabolism[MESH]
  • |Surface Plasmon Resonance/instrumentation/*methods[MESH]


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