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10.4155/bio-2020-0289 http://scihub22266oqcxt.onion/10.4155/bio-2020-0289 33319585!7739400!33319585     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Bioanalysis 2021 ; 13 (1): 13-28 Nephropedia Template TP {{tp|p=33319585|t=2021. Validation of dried blood spot sample modifications to two commercially available COVID-19 IgG antibody immunoassays |pdf=|usr=}}{{33319585}} gab.com Text Validation of dried blood spot sample modifications to two commercially available COVID-19 IgG antibody immunoassays JO: Bioanalysis http://www.kidney.de/pget.php?&tw=1&pmid=33319585 #FOAvip Aim: Coronavirus disease 2019 antibody testing often relies on venous blood collection, which is labor-intensive, inconvenient and expensive compared with finger-stick capillary dried blood spot (DBS) collection. The purpose of our work was to determine if two commercially available anti-severe acute respiratory syndrome coronavirus 2 enzyme-linked immunosorbent assays for IgG antibodies against spike S1 subunit and nucleocapsid proteins could be validated for use with DBS. Materials & methods: Kit supplied reagents were used to extract DBS, and in-house DBS calibrators were included on every run. Results: Positive/negative concordance between DBS and serum was 100/99.3% for the spike S1 subunit assay and 100/98% for the nucleocapsid assay. Conclusion: Validation of the DBS Coronavirus disease 2019 IgG antibody assays demonstrated that serum and DBS can produce equivalent results with minimal kit modifications. Twit Text FOAVip Validation of dried blood spot sample modifications to two commercially available COVID-19 IgG antibody immunoassays ????Bioanalysis http://www.kidney.de/pget.php?&tw=1&pmid=33319585 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33319585 #FOAvip English Wikipedia <ref>{{Cite pmid|33319585}}</ref> Validation of dried blood spot sample modifications to two commercially available COVID-19 IgG antibody immunoassays #MMPMID33319585 Zava TT; Zava DT Bioanalysis 2021[Jan]; 13 (1): 13-28 PMID33319585show ga Aim: Coronavirus disease 2019 antibody testing often relies on venous blood collection, which is labor-intensive, inconvenient and expensive compared with finger-stick capillary dried blood spot (DBS) collection. The purpose of our work was to determine if two commercially available anti-severe acute respiratory syndrome coronavirus 2 enzyme-linked immunosorbent assays for IgG antibodies against spike S1 subunit and nucleocapsid proteins could be validated for use with DBS. Materials & methods: Kit supplied reagents were used to extract DBS, and in-house DBS calibrators were included on every run. Results: Positive/negative concordance between DBS and serum was 100/99.3% for the spike S1 subunit assay and 100/98% for the nucleocapsid assay. Conclusion: Validation of the DBS Coronavirus disease 2019 IgG antibody assays demonstrated that serum and DBS can produce equivalent results with minimal kit modifications. |Antibodies, Viral/chemistry[MESH] |Antigens, Viral/blood/immunology[MESH] |COVID-19 Testing/*standards[MESH] |COVID-19/blood/*diagnosis/immunology/virology[MESH] |Coronavirus Nucleocapsid Proteins/blood/immunology[MESH] |Dried Blood Spot Testing/*standards[MESH] |Enzyme-Linked Immunosorbent Assay/*standards[MESH] |Female[MESH] |Humans[MESH] |Immunoglobulin G/chemistry[MESH] |Male[MESH] |Middle Aged[MESH] |Phosphoproteins/blood/immunology[MESH] |Reagent Kits, Diagnostic/standards[MESH] |Reproducibility of Results[MESH] |SARS-CoV-2/*immunology[MESH] |Sensitivity and Specificity[MESH]Validation of dried blood spot sample modifications to two commerciall(epmc).pdf DeepDyve Pubget Overpricing