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10.1093/labmed/lmaa101

http://scihub22266oqcxt.onion/10.1093/labmed/lmaa101
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33316059!7798980!33316059
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suck abstract from ncbi


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pmid33316059      Lab+Med 2021 ; 52 (2): 116-121
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  • CRISPR-Based Approaches for Efficient and Accurate Detection of SARS-CoV-2 #MMPMID33316059
  • Zhang W; Liu K; Zhang P; Cheng W; Li L; Zhang F; Yu Z; Li L; Zhang X
  • Lab Med 2021[Mar]; 52 (2): 116-121 PMID33316059show ga
  • An outbreak of COVID-19, caused by infection with SARS-CoV-2 in Wuhan, China in December 2019, spread throughout the country and around the world, quickly. The primary detection technique for SARS-CoV-2, the reverse-transcription polymerase chain reaction (RT-PCR)-based approach, requires expensive reagents and equipment and skilled personnel. In addition, for SARS-CoV-2 detection, specimens are usually shipped to a designated laboratory for testing, which may extend the diagnosis and treatment time of patients with COVID-19. The latest research shows that clustered regularly interspaced short palindromic repeats (CRISPR)-based approaches can quickly provide visual, rapid, ultrasensitive, and specific detection of SARS-CoV-2 at isothermal conditions. Therefore, CRISPR-based approaches are expected to be developed as attractive alternatives to conventional RT-PCR methods for the efficient and accurate detection of SARS-CoV-2. Recent advances in the field of CRISPR-based biosensing technologies for SARS-CoV-2 detection and insights into their potential use in many applications are reviewed in this article.
  • |*COVID-19 Nucleic Acid Testing[MESH]
  • |*Clustered Regularly Interspaced Short Palindromic Repeats[MESH]
  • |Humans[MESH]


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