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10.1002/cti2.1219

http://scihub22266oqcxt.onion/10.1002/cti2.1219
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33312565!7720530!33312565
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suck abstract from ncbi


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pmid33312565      Clin+Transl+Immunology 2020 ; 9 (12): e1219
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  • Rapid detection of SARS-CoV-2-specific memory T-cell immunity in recovered COVID-19 cases #MMPMID33312565
  • Lineburg KE; Srihari S; Altaf M; Swaminathan S; Panikkar A; Raju J; Crooks P; Ambalathingal GR; Martins JP; Matthews KK; Neller MA; Khanna R; Smith C
  • Clin Transl Immunology 2020[]; 9 (12): e1219 PMID33312565show ga
  • OBJECTIVES: There is emerging evidence that SARS-CoV-2-specific memory T-cell responses are likely to provide critical long-term protection against COVID-19. Strategies to rapidly assess T-cell responses are therefore likely to be important for assessing immunity in the global population. METHODS: Here, we have developed a rapid immune-monitoring strategy to assess virus-specific memory T-cell responses in the peripheral blood of COVID-19 convalescent individuals. We validated SARS-CoV-2-specific memory T-cell responses detected in whole blood using in vitro expansion with SARS-CoV-2 proteins. RESULTS: T-cell immunity characterised by the production of IFN-gamma and IL-2 could be consistently detected in the whole blood of recovered participants. T cells predominantly recognised structural SARS-CoV-2 proteins. In vitro expansion demonstrated that while CD8(+) T cells recognised nucleocapsid protein, spike protein and ORF3a, CD4(+) T cells more broadly targeted multiple SARS-CoV-2 proteins. CONCLUSION: These observations provide a timely monitoring approach for identifying SARS-CoV-2 cellular immunity and may serve as a diagnostic for the stratification of risk in immunocompromised and other at-risk individuals.
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