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10.1128/JCM.02880-20 http://scihub22266oqcxt.onion/10.1128/JCM.02880-20 33310764!8106729!33310764     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Clin+Microbiol 2021 ; 59 (3): ä Nephropedia Template TP {{tp|p=33310764|t=2021. Analytical Sensitivity of the Abbott BinaxNOW COVID-19 Ag Card |pdf=|usr=}}{{33310764}} gab.com Text Analytical Sensitivity of the Abbott BinaxNOW COVID-19 Ag Card JO: J Clin Microbiol http://www.kidney.de/pget.php?&tw=1&pmid=33310764 #FOAvip Multiple rapid antigen (Ag) tests for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have recently received emergency-use authorization (EUA) from the U.S. Food and Drug Administration (FDA). Although less sensitive than molecular detection methods, rapid antigen testing offers the potential for inexpensive, quick, decentralized testing. Robust analytical sensitivity data in comparison to reverse transcription-quantitative PCR (qRT-PCR) are currently lacking for many rapid antigen tests. Here, we evaluated the analytical sensitivity of the Abbott BinaxNOW COVID-19 Ag card using SARS-CoV-2-positive clinical specimens quantified by reverse transcription-droplet digital PCR (RT-ddPCR) and multiple FDA EUA qRT-PCR platforms using RNA standards. Initial and confirmatory limits of detection for the BinaxNOW COVID-19 Ag card were determined to be equivalent to 4.04 x 10(4) to 8.06 x 10(4) copies/swab. We further confirmed this limit of detection with 72 additional clinical samples positive for SARS-CoV-2 in either phosphate-buffered saline or viral transport medium. One hundred percent of samples with viral loads of >40,000 copies/swab were detected by rapid antigen testing. These data indicate that the BinaxNOW COVID-19 Ag card has an analytical sensitivity approximately equivalent to a generic qRT-PCR cycle threshold (C(T) ) value of 29 to 30. Twit Text FOAVip Analytical Sensitivity of the Abbott BinaxNOW COVID-19 Ag Card ????J Clin Microbiol http://www.kidney.de/pget.php?&tw=1&pmid=33310764 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33310764 #FOAvip English Wikipedia <ref>{{Cite pmid|33310764}}</ref> Analytical Sensitivity of the Abbott BinaxNOW COVID-19 Ag Card #MMPMID33310764 Perchetti GA; Huang ML; Mills MG; Jerome KR; Greninger AL J Clin Microbiol 2021[Feb]; 59 (3): ä PMID33310764show ga Multiple rapid antigen (Ag) tests for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have recently received emergency-use authorization (EUA) from the U.S. Food and Drug Administration (FDA). Although less sensitive than molecular detection methods, rapid antigen testing offers the potential for inexpensive, quick, decentralized testing. Robust analytical sensitivity data in comparison to reverse transcription-quantitative PCR (qRT-PCR) are currently lacking for many rapid antigen tests. Here, we evaluated the analytical sensitivity of the Abbott BinaxNOW COVID-19 Ag card using SARS-CoV-2-positive clinical specimens quantified by reverse transcription-droplet digital PCR (RT-ddPCR) and multiple FDA EUA qRT-PCR platforms using RNA standards. Initial and confirmatory limits of detection for the BinaxNOW COVID-19 Ag card were determined to be equivalent to 4.04 x 10(4) to 8.06 x 10(4) copies/swab. We further confirmed this limit of detection with 72 additional clinical samples positive for SARS-CoV-2 in either phosphate-buffered saline or viral transport medium. One hundred percent of samples with viral loads of >40,000 copies/swab were detected by rapid antigen testing. These data indicate that the BinaxNOW COVID-19 Ag card has an analytical sensitivity approximately equivalent to a generic qRT-PCR cycle threshold (C(T) ) value of 29 to 30. |*Specimen Handling[MESH] |COVID-19 Testing/methods/*standards[MESH] |COVID-19/*diagnosis[MESH] |Clinical Laboratory Techniques[MESH] |Humans[MESH] |Reverse Transcriptase Polymerase Chain Reaction[MESH] |SARS-CoV-2[MESH]Analytical Sensitivity of the Abbott BinaxNOW COVID-19 Ag Card (epmc).pdf DeepDyve Pubget Overpricing