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10.1126/sciadv.abe3703 http://scihub22266oqcxt.onion/10.1126/sciadv.abe3703 33310733!7793573!33310733     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Sci+Adv 2021 ; 7 (2): ä Nephropedia Template TP {{tp|p=33310733|t=2021. A smartphone-read ultrasensitive and quantitative saliva test for COVID-19 |pdf=|usr=}}{{33310733}} gab.com Text A smartphone-read ultrasensitive and quantitative saliva test for COVID-19 JO: Sci Adv http://www.kidney.de/pget.php?&tw=1&pmid=33310733 #FOAvip Point-of-care COVID-19 assays that are more sensitive than the current RT-PCR (reverse transcription polymerase chain reaction) gold standard assay are needed to improve disease control efforts. We describe the development of a portable, ultrasensitive saliva-based COVID-19 assay with a 15-min sample-to-answer time that does not require RNA isolation or laboratory equipment. This assay uses CRISPR-Cas12a activity to enhance viral amplicon signal, which is stimulated by the laser diode of a smartphone-based fluorescence microscope device. This device robustly quantified viral load over a broad linear range (1 to 10(5) copies/mul) and exhibited a limit of detection (0.38 copies/mul) below that of the RT-PCR reference assay. CRISPR-read SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2) RNA levels were similar in patient saliva and nasal swabs, and viral loads measured by RT-PCR and the smartphone-read CRISPR assay demonstrated good correlation, supporting the potential use of this portable assay for saliva-based point-of-care COVID-19 diagnosis. Twit Text FOAVip A smartphone-read ultrasensitive and quantitative saliva test for COVID-19 ????Sci Adv http://www.kidney.de/pget.php?&tw=1&pmid=33310733 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33310733 #FOAvip English Wikipedia <ref>{{Cite pmid|33310733}}</ref> A smartphone-read ultrasensitive and quantitative saliva test for COVID-19 #MMPMID33310733 Ning B; Yu T; Zhang S; Huang Z; Tian D; Lin Z; Niu A; Golden N; Hensley K; Threeton B; Lyon CJ; Yin XM; Roy CJ; Saba NS; Rappaport J; Wei Q; Hu TY Sci Adv 2021[Jan]; 7 (2): ä PMID33310733show ga Point-of-care COVID-19 assays that are more sensitive than the current RT-PCR (reverse transcription polymerase chain reaction) gold standard assay are needed to improve disease control efforts. We describe the development of a portable, ultrasensitive saliva-based COVID-19 assay with a 15-min sample-to-answer time that does not require RNA isolation or laboratory equipment. This assay uses CRISPR-Cas12a activity to enhance viral amplicon signal, which is stimulated by the laser diode of a smartphone-based fluorescence microscope device. This device robustly quantified viral load over a broad linear range (1 to 10(5) copies/mul) and exhibited a limit of detection (0.38 copies/mul) below that of the RT-PCR reference assay. CRISPR-read SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2) RNA levels were similar in patient saliva and nasal swabs, and viral loads measured by RT-PCR and the smartphone-read CRISPR assay demonstrated good correlation, supporting the potential use of this portable assay for saliva-based point-of-care COVID-19 diagnosis. |*COVID-19 Nucleic Acid Testing[MESH] |*Point-of-Care Testing[MESH] |*Smartphone[MESH] |Animals[MESH] |COVID-19/*diagnosis[MESH] |CRISPR-Cas Systems[MESH] |Chlorocebus aethiops[MESH] |Computer Simulation[MESH] |Female[MESH] |Humans[MESH] |Limit of Detection[MESH] |Macaca mulatta[MESH] |Male[MESH] |Molecular Diagnostic Techniques/instrumentation[MESH] |Reverse Transcriptase Polymerase Chain Reaction[MESH] |Saliva/*virology[MESH] |Sensitivity and Specificity[MESH] |Vero Cells[MESH]A smartphone-read ultrasensitive and quantitative saliva test for COVI(epmc).pdf DeepDyve Pubget Overpricing