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10.1016/j.lfs.2020.118889 http://scihub22266oqcxt.onion/10.1016/j.lfs.2020.118889 33310043!7834886!33310043     free     free     free Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Life+Sci 2021 ; 266 (ä): 118889 Nephropedia Template TP {{tp|p=33310043|t=2021. Screened antipsychotic drugs inhibit SARS-CoV-2 binding with ACE2 in vitro |pdf=|usr=}}{{33310043}} gab.com Text Screened antipsychotic drugs inhibit SARS-CoV-2 binding with ACE2 in vitro JO: Life Sci http://www.kidney.de/pget.php?&tw=1&pmid=33310043 #FOAvip AIM: The coronavirus disease 2019 (COVID-19) pandemic has swept the globe and no specific effective drug has been identified. Drug repurposing is a well-known method to address the crisis in a time-critical fashion. Antipsychotic drugs (APDs) have been reported to inhibit DNA replication of hepatitis B virus, measles virus germination, and HIV infection, along with replication of SARS-CoV and MERS-CoV, both of which interact with host cells as SARS-CoV-2. METHODS: Nineteen APDs were screened using ACE2-HEK293T cell membrane chromatography (ACE2-HEK293T/CMC). Cytotoxicity assay, coronavirus spike pseudotype virus entry assay, surface plasmon resonance, and virtual molecular docking were applied to detect affinity between ACE2 protein and drugs and a potential antiviral property of the screened compounds. KEY FINDINGS: After the CMC screening, 8 of the 19 APDs were well-retained on ACE2-HEK293T/CMC column and showed significant antiviral activities in vitro. Three quarters of them belong to phenothiazine and could significantly inhibit the entrance of coronavirus into ACE2-HEK293T cells. Aother two drugs, aripiprazole and tiapride, exhibited weaker inhibition. We selected five of the drugs for subsequent evaluation. All five showed similar affinity to ACE2 and virtual molecular docking demonstrated they bound with different amino acids respectively on ACE2 which SARS-CoV-2 binds to. SIGNIFICANCE: Eight APDs were screened for binding with ACE2, five of which demonstrated potential protective effects against SARS-CoV-2 through acting on ACE2. Although the five drugs have a weak ability to block SARS-CoV-2 with a single binding site, they may provide a synergistic effect in adjuvant therapy of COVID-19 infection. Twit Text FOAVip Screened antipsychotic drugs inhibit SARS-CoV-2 binding with ACE2 in vitro ????Life Sci http://www.kidney.de/pget.php?&tw=1&pmid=33310043 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33310043 #FOAvip English Wikipedia <ref>{{Cite pmid|33310043}}</ref> Screened antipsychotic drugs inhibit SARS-CoV-2 binding with ACE2 in vitro #MMPMID33310043 Lu J; Hou Y; Ge S; Wang X; Wang J; Hu T; Lv Y; He H; Wang C Life Sci 2021[Feb]; 266 (ä): 118889 PMID33310043show ga AIM: The coronavirus disease 2019 (COVID-19) pandemic has swept the globe and no specific effective drug has been identified. Drug repurposing is a well-known method to address the crisis in a time-critical fashion. Antipsychotic drugs (APDs) have been reported to inhibit DNA replication of hepatitis B virus, measles virus germination, and HIV infection, along with replication of SARS-CoV and MERS-CoV, both of which interact with host cells as SARS-CoV-2. METHODS: Nineteen APDs were screened using ACE2-HEK293T cell membrane chromatography (ACE2-HEK293T/CMC). Cytotoxicity assay, coronavirus spike pseudotype virus entry assay, surface plasmon resonance, and virtual molecular docking were applied to detect affinity between ACE2 protein and drugs and a potential antiviral property of the screened compounds. KEY FINDINGS: After the CMC screening, 8 of the 19 APDs were well-retained on ACE2-HEK293T/CMC column and showed significant antiviral activities in vitro. Three quarters of them belong to phenothiazine and could significantly inhibit the entrance of coronavirus into ACE2-HEK293T cells. Aother two drugs, aripiprazole and tiapride, exhibited weaker inhibition. We selected five of the drugs for subsequent evaluation. All five showed similar affinity to ACE2 and virtual molecular docking demonstrated they bound with different amino acids respectively on ACE2 which SARS-CoV-2 binds to. SIGNIFICANCE: Eight APDs were screened for binding with ACE2, five of which demonstrated potential protective effects against SARS-CoV-2 through acting on ACE2. Although the five drugs have a weak ability to block SARS-CoV-2 with a single binding site, they may provide a synergistic effect in adjuvant therapy of COVID-19 infection. |Angiotensin-Converting Enzyme 2/antagonists & inhibitors/chemistry/*metabolism[MESH] |Antipsychotic Agents/chemistry/metabolism/*pharmacology[MESH] |Antiviral Agents/*pharmacology[MESH] |Cell Membrane[MESH] |Cell Survival/drug effects[MESH] |Chromatography, Liquid/methods[MESH] |Drug Evaluation, Preclinical/*methods[MESH] |Drug Repositioning[MESH] |HEK293 Cells[MESH] |Host-Pathogen Interactions/drug effects[MESH] |Humans[MESH] |Molecular Docking Simulation[MESH] |SARS-CoV-2/*drug effects/pathogenicity[MESH] |Spike Glycoprotein, Coronavirus/metabolism[MESH] |Surface Plasmon Resonance[MESH]Screened antipsychotic drugs inhibit SARS-CoV-2 binding with ACE2 in v(epmc).pdf DeepDyve Pubget Overpricing