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10.1016/j.ijid.2020.12.017

http://scihub22266oqcxt.onion/10.1016/j.ijid.2020.12.017
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33310021!7726521!33310021
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suck abstract from ncbi


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pmid33310021      Int+J+Infect+Dis 2021 ; 103 (ä): 381-388
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  • Comparison of six commercially available SARS-CoV-2 antibody assays-Choice of assay depends on intended use #MMPMID33310021
  • Nilsson AC; Holm DK; Justesen US; Gorm-Jensen T; Andersen NS; Ovrehus A; Johansen IS; Michelsen J; Sprogoe U; Lillevang ST
  • Int J Infect Dis 2021[Feb]; 103 (ä): 381-388 PMID33310021show ga
  • OBJECTIVES: Evaluate six commercial serological assays for detection of IgA, IgM or IgG SARS-CoV-2 antibodies in different disease severities. METHODS: Three lateral flow tests (LFTs) (Acro IgM/IgG, CTK IgM/IgG, Livzon IgM/IgG) and three ELISA assays (Euroimmun IgA and IgG, Wantai IgM) were included. Application was evaluated using samples from 57 patients with a positive SARS-CoV-2 reverse transcription polymerase chain reaction, stratified according to disease severity. Specificity was assessed using historical samples from 200 blood donors. RESULTS: While IgM LFTs failed to detect SARS-CoV-2 antibodies in 37-84% of non-hospitalised patients, the Wantai IgM ELISA detected antibodies in 79%. The Euroimmun IgG ELISA detected antibodies in 95% of non-hospitalised patients. IgA, IgM and IgG ELISA levels were initially low, increased over time, and correlated with disease severity. LFT sensitivity declined in samples taken >28 days after symptom onset/resolution. The Livzon IgG LFT had the highest specificity (98.5%), followed by the Euroimmun IgG ELISA (96.2%). The specificity for Euroimmun IgA ELISA improved (>/=97.5%) using a custom cut-off value (4.0). CONCLUSIONS: The sensitive and semi-quantitative ELISA assays are most appropriate for serologic detection of SARS-CoV-2 infection in mild cases. Livzon LFT and Euroimmun ELISA had the highest specificity among the IgG assays, making them most suitable for seroprevalence studies.
  • |Adult[MESH]
  • |Antibodies, Viral/*blood[MESH]
  • |COVID-19 Serological Testing/*methods[MESH]
  • |COVID-19/*diagnosis[MESH]
  • |Enzyme-Linked Immunosorbent Assay[MESH]
  • |Female[MESH]
  • |Humans[MESH]
  • |Immunoglobulin G/blood[MESH]
  • |Immunoglobulin M/blood[MESH]
  • |Male[MESH]
  • |Middle Aged[MESH]
  • |SARS-CoV-2[MESH]


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