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10.1016/j.cell.2020.12.001 http://scihub22266oqcxt.onion/10.1016/j.cell.2020.12.001 33306959!7834310!33306959     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Cell 2021 ; 184 (2): 323-333.e9 Nephropedia Template TP {{tp|p=33306959|t=2021. Amplification-free detection of SARS-CoV-2 with CRISPR-Cas13a and mobile phone microscopy |pdf=|usr=}}{{33306959}} gab.com Text Amplification-free detection of SARS-CoV-2 with CRISPR-Cas13a and mobile phone microscopy JO: Cell http://www.kidney.de/pget.php?&tw=1&pmid=33306959 #FOAvip The December 2019 outbreak of a novel respiratory virus, SARS-CoV-2, has become an ongoing global pandemic due in part to the challenge of identifying symptomatic, asymptomatic, and pre-symptomatic carriers of the virus. CRISPR diagnostics can augment gold-standard PCR-based testing if they can be made rapid, portable, and accurate. Here, we report the development of an amplification-free CRISPR-Cas13a assay for direct detection of SARS-CoV-2 from nasal swab RNA that can be read with a mobile phone microscope. The assay achieved approximately 100 copies/muL sensitivity in under 30 min of measurement time and accurately detected pre-extracted RNA from a set of positive clinical samples in under 5 min. We combined crRNAs targeting SARS-CoV-2 RNA to improve sensitivity and specificity and directly quantified viral load using enzyme kinetics. Integrated with a reader device based on a mobile phone, this assay has the potential to enable rapid, low-cost, point-of-care screening for SARS-CoV-2. Twit Text FOAVip Amplification-free detection of SARS-CoV-2 with CRISPR-Cas13a and mobile phone microscopy ????Cell http://www.kidney.de/pget.php?&tw=1&pmid=33306959 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33306959 #FOAvip English Wikipedia <ref>{{Cite pmid|33306959}}</ref> Amplification-free detection of SARS-CoV-2 with CRISPR-Cas13a and mobile phone microscopy #MMPMID33306959 Fozouni P; Son S; Diaz de Leon Derby M; Knott GJ; Gray CN; D'Ambrosio MV; Zhao C; Switz NA; Kumar GR; Stephens SI; Boehm D; Tsou CL; Shu J; Bhuiya A; Armstrong M; Harris AR; Chen PY; Osterloh JM; Meyer-Franke A; Joehnk B; Walcott K; Sil A; Langelier C; Pollard KS; Crawford ED; Puschnik AS; Phelps M; Kistler A; DeRisi JL; Doudna JA; Fletcher DA; Ott M Cell 2021[Jan]; 184 (2): 323-333.e9 PMID33306959show ga The December 2019 outbreak of a novel respiratory virus, SARS-CoV-2, has become an ongoing global pandemic due in part to the challenge of identifying symptomatic, asymptomatic, and pre-symptomatic carriers of the virus. CRISPR diagnostics can augment gold-standard PCR-based testing if they can be made rapid, portable, and accurate. Here, we report the development of an amplification-free CRISPR-Cas13a assay for direct detection of SARS-CoV-2 from nasal swab RNA that can be read with a mobile phone microscope. The assay achieved approximately 100 copies/muL sensitivity in under 30 min of measurement time and accurately detected pre-extracted RNA from a set of positive clinical samples in under 5 min. We combined crRNAs targeting SARS-CoV-2 RNA to improve sensitivity and specificity and directly quantified viral load using enzyme kinetics. Integrated with a reader device based on a mobile phone, this assay has the potential to enable rapid, low-cost, point-of-care screening for SARS-CoV-2. |Animals[MESH] |COVID-19 Nucleic Acid Testing/economics/instrumentation/*methods[MESH] |CRISPR-Cas Systems[MESH] |Cell Line[MESH] |Cell Phone/*instrumentation[MESH] |Coronavirus Nucleocapsid Proteins/genetics[MESH] |Humans[MESH] |Nasopharynx/virology[MESH] |Optical Imaging/instrumentation/*methods[MESH] |Phosphoproteins/genetics[MESH] |Point-of-Care Testing[MESH] |RNA Interference[MESH] |RNA, Viral/*analysis/genetics[MESH] |Sensitivity and Specificity[MESH]Amplification-free detection of SARS-CoV-2 with CRISPR-Cas13a and mobi(epmc).pdf DeepDyve Pubget Overpricing