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10.1002/jcp.30206 http://scihub22266oqcxt.onion/10.1002/jcp.30206 33305832!ä!33305832 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Cell+Physiol 2021 ; 236 (7): 4966-4972 Nephropedia Template TP {{tp|p=33305832|t=2021. Thermal inactivation of COVID-19 specimens improves RNA quality and quantity |pdf=|usr=}}{{33305832}} gab.com Text Thermal inactivation of COVID-19 specimens improves RNA quality and quantity JO: J Cell Physiol http://www.kidney.de/pget.php?&tw=1&pmid=33305832 #FOAvip The rapid spread of coronavirus disease 2019 (COVID-19), a disease caused by severe acute respiratory syndrome coronavirus 2, poses a huge demand for immediate diagnosis. Real-time reverse transcriptase-polymerase chain reaction (rRT-PCR) of nasopharyngeal (NP) and oropharyngeal (OP) swabs have been used to confirm the clinical diagnosis. To avoid the risk of viral-exposure of laboratory workers, thermal inactivation is currently recommended but has unknown effects on the accuracy of the rRT-PCR results. Thirty-six NP/OP specimens were collected from COVID-19 patients and subjected to thermal inactivation (60 degrees C for 30 min) or the RNA extraction processes to activate the form. Here, our data showed that the concentration of extracted-RNA increases upon thermal inactivation compared to the active form (p = .028). Significantly higher levels of RNA copy number were obtained in inactivated compared to the active samples for both N and ORF1ab genes (p = .009, p = .032, respectively). Thermal inactivation elevated concentration and copy number of extracted-RNA, possibly through viral-capsid degradation and/or nucleoprotein denaturation. Twit Text FOAVip Thermal inactivation of COVID-19 specimens improves RNA quality and quantity ????J Cell Physiol http://www.kidney.de/pget.php?&tw=1&pmid=33305832 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33305832 #FOAvip English Wikipedia <ref>{{Cite pmid|33305832}}</ref> Thermal inactivation of COVID-19 specimens improves RNA quality and quantity #MMPMID33305832 Hemati M; Soosanabadi M; Ghorashi T; Ghaffari H; Vahedi A; Sabbaghian E; Rasouli Nejad Z; Salati A; Danaei N; Kokhaei P J Cell Physiol 2021[Jul]; 236 (7): 4966-4972 PMID33305832show ga The rapid spread of coronavirus disease 2019 (COVID-19), a disease caused by severe acute respiratory syndrome coronavirus 2, poses a huge demand for immediate diagnosis. Real-time reverse transcriptase-polymerase chain reaction (rRT-PCR) of nasopharyngeal (NP) and oropharyngeal (OP) swabs have been used to confirm the clinical diagnosis. To avoid the risk of viral-exposure of laboratory workers, thermal inactivation is currently recommended but has unknown effects on the accuracy of the rRT-PCR results. Thirty-six NP/OP specimens were collected from COVID-19 patients and subjected to thermal inactivation (60 degrees C for 30 min) or the RNA extraction processes to activate the form. Here, our data showed that the concentration of extracted-RNA increases upon thermal inactivation compared to the active form (p = .028). Significantly higher levels of RNA copy number were obtained in inactivated compared to the active samples for both N and ORF1ab genes (p = .009, p = .032, respectively). Thermal inactivation elevated concentration and copy number of extracted-RNA, possibly through viral-capsid degradation and/or nucleoprotein denaturation. |*COVID-19 Testing/statistics & numerical data[MESH] |*Clinical Laboratory Techniques/methods[MESH] |Adult[MESH] |Aged[MESH] |Aged, 80 and over[MESH] |COVID-19/*diagnosis/genetics[MESH] |Female[MESH] |Humans[MESH] |Male[MESH] |Middle Aged[MESH] |Nasopharynx/chemistry/virology[MESH] |RNA, Viral/*genetics[MESH] |Real-Time Polymerase Chain Reaction/methods[MESH]Thermal inactivation of COVID-19 specimens improves RNA quality and qu(epmc).pdf DeepDyve Pubget Overpricing