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10.1038/s41421-020-00222-5 http://scihub22266oqcxt.onion/10.1038/s41421-020-00222-5 33298900!7610239!33298900     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Cell+Discov 2020 ; 6 (1): 80 Nephropedia Template TP {{tp|p=33298900|t=2020. Heparan sulfate assists SARS-CoV-2 in cell entry and can be targeted by approved drugs in vitro |pdf=|usr=}}{{33298900}} gab.com Text Heparan sulfate assists SARS-CoV-2 in cell entry and can be targeted by approved drugs in vitro JO: Cell Discov http://www.kidney.de/pget.php?&tw=1&pmid=33298900 #FOAvip The cell entry of SARS-CoV-2 has emerged as an attractive drug repurposing target for COVID-19. Here we combine genetics and chemical perturbation to demonstrate that ACE2-mediated entry of SARS-Cov and CoV-2 requires the cell surface heparan sulfate (HS) as an assisting cofactor: ablation of genes involved in HS biosynthesis or incubating cells with a HS mimetic both inhibit Spike-mediated viral entry. We show that heparin/HS binds to Spike directly, and facilitates the attachment of Spike-bearing viral particles to the cell surface to promote viral entry. We screened approved drugs and identified two classes of inhibitors that act via distinct mechanisms to target this entry pathway. Among the drugs characterized, Mitoxantrone is a potent HS inhibitor, while Sunitinib and BNTX disrupt the actin network to indirectly abrogate HS-assisted viral entry. We further show that drugs of the two classes can be combined to generate a synergized activity against SARS-CoV-2-induced cytopathic effect. Altogether, our study establishes HS as an attachment factor that assists SARS coronavirus cell entry and reveals drugs capable of targeting this important step in the viral life cycle. Twit Text FOAVip Heparan sulfate assists SARS-CoV-2 in cell entry and can be targeted by approved drugs in vitro ????Cell Discov http://www.kidney.de/pget.php?&tw=1&pmid=33298900 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33298900 #FOAvip English Wikipedia <ref>{{Cite pmid|33298900}}</ref> Heparan sulfate assists SARS-CoV-2 in cell entry and can be targeted by approved drugs in vitro #MMPMID33298900 Zhang Q; Chen CZ; Swaroop M; Xu M; Wang L; Lee J; Wang AQ; Pradhan M; Hagen N; Chen L; Shen M; Luo Z; Xu X; Xu Y; Huang W; Zheng W; Ye Y Cell Discov 2020[Nov]; 6 (1): 80 PMID33298900show ga The cell entry of SARS-CoV-2 has emerged as an attractive drug repurposing target for COVID-19. Here we combine genetics and chemical perturbation to demonstrate that ACE2-mediated entry of SARS-Cov and CoV-2 requires the cell surface heparan sulfate (HS) as an assisting cofactor: ablation of genes involved in HS biosynthesis or incubating cells with a HS mimetic both inhibit Spike-mediated viral entry. We show that heparin/HS binds to Spike directly, and facilitates the attachment of Spike-bearing viral particles to the cell surface to promote viral entry. We screened approved drugs and identified two classes of inhibitors that act via distinct mechanisms to target this entry pathway. Among the drugs characterized, Mitoxantrone is a potent HS inhibitor, while Sunitinib and BNTX disrupt the actin network to indirectly abrogate HS-assisted viral entry. We further show that drugs of the two classes can be combined to generate a synergized activity against SARS-CoV-2-induced cytopathic effect. Altogether, our study establishes HS as an attachment factor that assists SARS coronavirus cell entry and reveals drugs capable of targeting this important step in the viral life cycle. äHeparan sulfate assists SARS-CoV-2 in cell entry and can be targeted b(epmc).pdf DeepDyve Pubget Overpricing