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10.1080/22221751.2020.1861914 http://scihub22266oqcxt.onion/10.1080/22221751.2020.1861914 33295839!7782039!33295839     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Emerg+Microbes+Infect 2020 ; 9 (1): 2714-2726 Nephropedia Template TP {{tp|p=33295839|t=2020. An infectious cDNA clone of a growth attenuated Korean isolate of MERS coronavirus KNIH002 in clade B |pdf=|usr=}}{{33295839}} gab.com Text An infectious cDNA clone of a growth attenuated Korean isolate of MERS coronavirus KNIH002 in clade B JO: Emerg Microbes Infect http://www.kidney.de/pget.php?&tw=1&pmid=33295839 #FOAvip The MERS-CoV isolated during the 2015 nosocomial outbreak in Korea showed distinctive differences in mortality and transmission patterns compared to the prototype MERS-CoV EMC strain belonging to clade A. We established a BAC-based reverse genetics system for a Korean isolate of MERS-CoV KNIH002 in the clade B phylogenetically far from the EMC strain, and generated a recombinant MERS-CoV expressing red fluorescent protein. The virus rescued from the infectious clone and KNIH002 strain displayed growth attenuation compared to the EMC strain. Consecutive passages of the rescued virus rapidly generated various ORF5 variants, highlighting its genetic instability and calling for caution in the use of repeatedly passaged virus in pathogenesis studies and for evaluation of control measures against MERS-CoV. The infectious clone for the KNIH002 in contemporary epidemic clade B would be useful for better understanding of a functional link between molecular evolution and pathophysiology of MERS-CoV by comparative studies with EMC strain. Twit Text FOAVip An infectious cDNA clone of a growth attenuated Korean isolate of MERS coronavirus KNIH002 in clade B ????Emerg Microbes Infect http://www.kidney.de/pget.php?&tw=1&pmid=33295839 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33295839 #FOAvip English Wikipedia <ref>{{Cite pmid|33295839}}</ref> An infectious cDNA clone of a growth attenuated Korean isolate of MERS coronavirus KNIH002 in clade B #MMPMID33295839 Kim M; Cho H; Lee SH; Park WJ; Kim JM; Moon JS; Kim GW; Lee W; Jung HG; Yang JS; Choi JH; Lee JY; Kim SS; Oh JW Emerg Microbes Infect 2020[Dec]; 9 (1): 2714-2726 PMID33295839show ga The MERS-CoV isolated during the 2015 nosocomial outbreak in Korea showed distinctive differences in mortality and transmission patterns compared to the prototype MERS-CoV EMC strain belonging to clade A. We established a BAC-based reverse genetics system for a Korean isolate of MERS-CoV KNIH002 in the clade B phylogenetically far from the EMC strain, and generated a recombinant MERS-CoV expressing red fluorescent protein. The virus rescued from the infectious clone and KNIH002 strain displayed growth attenuation compared to the EMC strain. Consecutive passages of the rescued virus rapidly generated various ORF5 variants, highlighting its genetic instability and calling for caution in the use of repeatedly passaged virus in pathogenesis studies and for evaluation of control measures against MERS-CoV. The infectious clone for the KNIH002 in contemporary epidemic clade B would be useful for better understanding of a functional link between molecular evolution and pathophysiology of MERS-CoV by comparative studies with EMC strain. |Animals[MESH] |Cell Line, Tumor[MESH] |Chlorocebus aethiops[MESH] |Clone Cells[MESH] |Cricetinae[MESH] |DNA, Complementary/*toxicity[MESH] |Humans[MESH] |Middle East Respiratory Syndrome Coronavirus/*genetics/growth & development[MESH] |Receptors, Virus/metabolism[MESH] |Vero Cells[MESH]An infectious cDNA clone of a growth attenuated Korean isolate of MERS(epmc).pdf DeepDyve Pubget Overpricing