Use my Search Websuite to scan PubMed, PMCentral, Journal Hosts and Journal Archives, FullText. Kick-your-searchterm to multiple Engines kick-your-query now !>

A dictionary by aggregated review articles of nephrology, medicine and the life sciences Your one-stop-run pathway from word to the immediate pdf of peer-reviewed on-topic knowledge.

10.1093/jmcb/mjaa067 http://scihub22266oqcxt.onion/10.1093/jmcb/mjaa067 33295606!7799037!33295606     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=33295606&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\33295606.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       J+Mol+Cell+Biol 2020 ; 12 (12): 916-932 Nephropedia Template TP {{tp|p=33295606|t=2020. SARS-CoV-2 spike protein binds to bacterial lipopolysaccharide and boosts proinflammatory activity |pdf=|usr=}}{{33295606}} gab.com Text SARS-CoV-2 spike protein binds to bacterial lipopolysaccharide and boosts proinflammatory activity JO: J Mol Cell Biol http://www.kidney.de/pget.php?&tw=1&pmid=33295606 #FOAvip There is a link between high lipopolysaccharide (LPS) levels in the blood and the metabolic syndrome, and metabolic syndrome predisposes patients to severe COVID-19. Here, we define an interaction between SARS-CoV-2 spike (S) protein and LPS, leading to aggravated inflammation in vitro and in vivo. Native gel electrophoresis demonstrated that SARS-CoV-2 S protein binds to LPS. Microscale thermophoresis yielded a KD of approximately 47 nM for the interaction. Computational modeling and all-atom molecular dynamics simulations further substantiated the experimental results, identifying a main LPS-binding site in SARS-CoV-2 S protein. S protein, when combined with low levels of LPS, boosted nuclear factor-kappa B (NF-kappaB) activation in monocytic THP-1 cells and cytokine responses in human blood and peripheral blood mononuclear cells, respectively. The in vitro inflammatory response was further validated by employing NF-kappaB reporter mice and in vivo bioimaging. Dynamic light scattering, transmission electron microscopy, and LPS-FITC analyses demonstrated that S protein modulated the aggregation state of LPS, providing a molecular explanation for the observed boosting effect. Taken together, our results provide an interesting molecular link between excessive inflammation during infection with SARS-CoV-2 and comorbidities involving increased levels of bacterial endotoxins. Twit Text FOAVip SARS-CoV-2 spike protein binds to bacterial lipopolysaccharide and boosts proinflammatory activity ????J Mol Cell Biol http://www.kidney.de/pget.php?&tw=1&pmid=33295606 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33295606 #FOAvip English Wikipedia <ref>{{Cite pmid|33295606}}</ref> SARS-CoV-2 spike protein binds to bacterial lipopolysaccharide and boosts proinflammatory activity #MMPMID33295606 Petruk G; Puthia M; Petrlova J; Samsudin F; Stromdahl AC; Cerps S; Uller L; Kjellstrom S; Bond PJ; Schmidtchen AA J Mol Cell Biol 2020[Oct]; 12 (12): 916-932 PMID33295606show ga There is a link between high lipopolysaccharide (LPS) levels in the blood and the metabolic syndrome, and metabolic syndrome predisposes patients to severe COVID-19. Here, we define an interaction between SARS-CoV-2 spike (S) protein and LPS, leading to aggravated inflammation in vitro and in vivo. Native gel electrophoresis demonstrated that SARS-CoV-2 S protein binds to LPS. Microscale thermophoresis yielded a KD of approximately 47 nM for the interaction. Computational modeling and all-atom molecular dynamics simulations further substantiated the experimental results, identifying a main LPS-binding site in SARS-CoV-2 S protein. S protein, when combined with low levels of LPS, boosted nuclear factor-kappa B (NF-kappaB) activation in monocytic THP-1 cells and cytokine responses in human blood and peripheral blood mononuclear cells, respectively. The in vitro inflammatory response was further validated by employing NF-kappaB reporter mice and in vivo bioimaging. Dynamic light scattering, transmission electron microscopy, and LPS-FITC analyses demonstrated that S protein modulated the aggregation state of LPS, providing a molecular explanation for the observed boosting effect. Taken together, our results provide an interesting molecular link between excessive inflammation during infection with SARS-CoV-2 and comorbidities involving increased levels of bacterial endotoxins. |*SARS-CoV-2/immunology/pathogenicity/physiology[MESH] |Animals[MESH] |Binding Sites[MESH] |COVID-19/*complications/immunology/virology[MESH] |Cytokine Release Syndrome/etiology/immunology[MESH] |Disease Models, Animal[MESH] |Gram-Negative Bacterial Infections/complications/immunology[MESH] |Humans[MESH] |In Vitro Techniques[MESH] |Inflammation/*etiology[MESH] |Lipid A/chemistry/immunology/metabolism[MESH] |Lipopolysaccharides/chemistry/immunology/*metabolism[MESH] |Mice[MESH] |Mice, Inbred BALB C[MESH] |Mice, Transgenic[MESH] |Models, Immunological[MESH] |Models, Molecular[MESH] |Molecular Docking Simulation[MESH] |Protein Binding[MESH] |Protein Interaction Domains and Motifs[MESH] |Respiratory Distress Syndrome/etiology[MESH] |Risk Factors[MESH]SARS-CoV-2 spike protein binds to bacterial lipopolysaccharide and boo(epmc).pdf DeepDyve Pubget Overpricing