Use my Search Websuite to scan PubMed, PMCentral, Journal Hosts and Journal Archives, FullText. Kick-your-searchterm to multiple Engines kick-your-query now !>

A dictionary by aggregated review articles of nephrology, medicine and the life sciences Your one-stop-run pathway from word to the immediate pdf of peer-reviewed on-topic knowledge.

10.11604/pamj.supp.2020.35.24258 http://scihub22266oqcxt.onion/10.11604/pamj.supp.2020.35.24258 33282076!7687508!33282076     free     free     free Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Pan+Afr+Med+J 2020 ; 35 (Suppl 2): 121 Nephropedia Template TP {{tp|p=33282076|t=2020. Real-time RT-PCR for COVID-19 diagnosis: challenges and prospects |pdf=|usr=}}{{33282076}} gab.com Text Real-time RT-PCR for COVID-19 diagnosis: challenges and prospects JO: Pan Afr Med J http://www.kidney.de/pget.php?&tw=1&pmid=33282076 #FOAvip COVID-19 impacts global public health, economy, education, tourism/hospitality and sports; rapid and accurate testing of clinical samples dictate effective response. So far, the real-time reverse transcriptase-polymerase chain reaction (RT-qPCR) is the assay of choice for COVID-19 diagnosis considering its rapidity and accuracy in informing on active coronavirus (CoV) infection. Presently, several RT-qPCR protocols with differing sensitivity/specificity are used for performing this assay; some of them are known to have generated debatable test results to constitute challenges worthy of consideration. This review provides a critical assessment of various published works on RT-qPCR assays used for COVID-19 diagnosis with their different indicators of positivity i.e., cycle threshold (Ct) cut-off values. Knowledge of diagnostic tests for COVID-19 is still evolving and, as a prospect, underscores the need for local validation of positive-negative Ct cut-off values when establishing RT-qPCR assays for SARS-CoV-2 detection. Twit Text FOAVip Real-time RT-PCR for COVID-19 diagnosis: challenges and prospects ????Pan Afr Med J http://www.kidney.de/pget.php?&tw=1&pmid=33282076 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33282076 #FOAvip English Wikipedia <ref>{{Cite pmid|33282076}}</ref> Real-time RT-PCR for COVID-19 diagnosis: challenges and prospects #MMPMID33282076 Sule WF; Oluwayelu DO Pan Afr Med J 2020[]; 35 (Suppl 2): 121 PMID33282076show ga COVID-19 impacts global public health, economy, education, tourism/hospitality and sports; rapid and accurate testing of clinical samples dictate effective response. So far, the real-time reverse transcriptase-polymerase chain reaction (RT-qPCR) is the assay of choice for COVID-19 diagnosis considering its rapidity and accuracy in informing on active coronavirus (CoV) infection. Presently, several RT-qPCR protocols with differing sensitivity/specificity are used for performing this assay; some of them are known to have generated debatable test results to constitute challenges worthy of consideration. This review provides a critical assessment of various published works on RT-qPCR assays used for COVID-19 diagnosis with their different indicators of positivity i.e., cycle threshold (Ct) cut-off values. Knowledge of diagnostic tests for COVID-19 is still evolving and, as a prospect, underscores the need for local validation of positive-negative Ct cut-off values when establishing RT-qPCR assays for SARS-CoV-2 detection. |*Real-Time Polymerase Chain Reaction[MESH] |*SARS-CoV-2[MESH] |COVID-19/*diagnosis[MESH] |Humans[MESH]Real-time RT-PCR for COVID-19 diagnosis: challenges and prospects (epmc).pdf DeepDyve Pubget Overpricing