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10.1126/sciadv.abc5546 http://scihub22266oqcxt.onion/10.1126/sciadv.abc5546 33277250!7821905!33277250     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=33277250&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215       Sci+Adv 2020 ; 6 (49): ? Nephropedia Template TP {{tp|p=33277250|t=2020. Using migrating cells as probes to illuminate features in live embryonic tissues |pdf=|usr=}}{{33277250}} gab.com Text Using migrating cells as probes to illuminate features in live embryonic tissues JO: Sci Adv http://www.kidney.de/pget.php?&tw=1&pmid=33277250 #FOAvip The biophysical and biochemical properties of live tissues are important in the context of development and disease. Methods for evaluating these properties typically involve destroying the tissue or require specialized technology and complicated analyses. Here, we present a novel, noninvasive methodology for determining the spatial distribution of tissue features within embryos, making use of nondirectionally migrating cells and software we termed "Landscape," which performs automatized high-throughput three-dimensional image registration. Using the live migrating cells as bioprobes, we identified structures within the zebrafish embryo that affect the distribution of the cells and studied one such structure constituting a physical barrier, which, in turn, influences amoeboid cell polarity. Overall, this work provides a unique approach for detecting tissue properties without interfering with animal's development. In addition, Landscape allows for integrating data from multiple samples, providing detailed and reliable quantitative evaluation of variable biological phenotypes in different organisms. Twit Text FOAVip Using migrating cells as probes to illuminate features in live embryonic tissues ????Sci Adv http://www.kidney.de/pget.php?&tw=1&pmid=33277250 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33277250 #FOAvip English Wikipedia <ref>{{Cite pmid|33277250}}</ref> Using migrating cells as probes to illuminate features in live embryonic tissues #MMPMID33277250 Gross-Thebing S; Truszkowski L; Tenbrinck D; Sanchez-Iranzo H; Camelo C; Westerich KJ; Singh A; Maier P; Prengel J; Lange P; Huwel J; Gaede F; Sasse R; Vos BE; Betz T; Matis M; Prevedel R; Luschnig S; Diz-Munoz A; Burger M; Raz E Sci Adv 2020[Dec]; 6 (49): ? PMID33277250show ga The biophysical and biochemical properties of live tissues are important in the context of development and disease. Methods for evaluating these properties typically involve destroying the tissue or require specialized technology and complicated analyses. Here, we present a novel, noninvasive methodology for determining the spatial distribution of tissue features within embryos, making use of nondirectionally migrating cells and software we termed "Landscape," which performs automatized high-throughput three-dimensional image registration. Using the live migrating cells as bioprobes, we identified structures within the zebrafish embryo that affect the distribution of the cells and studied one such structure constituting a physical barrier, which, in turn, influences amoeboid cell polarity. Overall, this work provides a unique approach for detecting tissue properties without interfering with animal's development. In addition, Landscape allows for integrating data from multiple samples, providing detailed and reliable quantitative evaluation of variable biological phenotypes in different organisms. |*Cell Polarity[MESH] |*Zebrafish/genetics[MESH]Using migrating cells as probes to illuminate features in live embryon(epmc).pdf DeepDyve Pubget Overpricing