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10.1016/j.jviromet.2020.114030 http://scihub22266oqcxt.onion/10.1016/j.jviromet.2020.114030 33275927!7706421!33275927     free     free     free Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Virol+Methods 2021 ; 288 (ä): 114030 Nephropedia Template TP {{tp|p=33275927|t=2021. Comparison of three TaqMan real-time reverse transcription-PCR assays in detecting SARS-CoV-2 |pdf=|usr=}}{{33275927}} gab.com Text Comparison of three TaqMan real-time reverse transcription-PCR assays in detecting SARS-CoV-2 JO: J Virol Methods http://www.kidney.de/pget.php?&tw=1&pmid=33275927 #FOAvip Quick and accurate detection of SARS-CoV-2 is critical for COVID-19 control. Dozens of real-time reverse transcription PCR (qRT-PCR) assays have been developed to meet the urgent need of COVID-19 control. However, methodological comparisons among the developed qRT-PCR assays are limited. In the present study, we evaluated the sensitivity, specificity, amplification efficiency, and linear detection ranges of three qRT-PCR assays, including the assays developed by our group (IPBCAMS), and the assays recommended by WHO and China CDC (CCDC). The three qRT-PCR assays exhibited similar sensitivities, with the limit of detection (LoD) at about 10 copies per reaction (except the ORF 1b gene assay in CCDC assays with a LoD at about 100 copies per reaction). No cross reaction with other respiratory viruses were observed in all of the three qRT-PCR assays. Wide linear detection ranges from 10(6) to 10(1) copies per reaction and acceptable reproducibility were obtained. By using 25 clinical specimens, the N gene assay of IPBCAMS assays and CCDC assays performed better (with detection rates of 92 % and 100 %, respectively) than that of the WHO assays (with a detection rate of 60 %), and the ORF 1b gene assay in IPBCAMS assays performed better (with a detection rate of 64 %) than those of the WHO assays and the CCDC assays (with detection rates of 48 % and 20 %, respectively). In conclusion, the N gene assays of CCDC assays and IPBCAMS assays and the ORF 1b gene assay of IPBCAMS assays were recommended for qRT-PCR screening of SARS-CoV-2. Twit Text FOAVip Comparison of three TaqMan real-time reverse transcription-PCR assays in detecting SARS-CoV-2 ????J Virol Methods http://www.kidney.de/pget.php?&tw=1&pmid=33275927 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33275927 #FOAvip English Wikipedia <ref>{{Cite pmid|33275927}}</ref> Comparison of three TaqMan real-time reverse transcription-PCR assays in detecting SARS-CoV-2 #MMPMID33275927 Xiao Y; Li Z; Wang X; Wang Y; Wang Y; Wang G; Ren L; Li J J Virol Methods 2021[Feb]; 288 (ä): 114030 PMID33275927show ga Quick and accurate detection of SARS-CoV-2 is critical for COVID-19 control. Dozens of real-time reverse transcription PCR (qRT-PCR) assays have been developed to meet the urgent need of COVID-19 control. However, methodological comparisons among the developed qRT-PCR assays are limited. In the present study, we evaluated the sensitivity, specificity, amplification efficiency, and linear detection ranges of three qRT-PCR assays, including the assays developed by our group (IPBCAMS), and the assays recommended by WHO and China CDC (CCDC). The three qRT-PCR assays exhibited similar sensitivities, with the limit of detection (LoD) at about 10 copies per reaction (except the ORF 1b gene assay in CCDC assays with a LoD at about 100 copies per reaction). No cross reaction with other respiratory viruses were observed in all of the three qRT-PCR assays. Wide linear detection ranges from 10(6) to 10(1) copies per reaction and acceptable reproducibility were obtained. By using 25 clinical specimens, the N gene assay of IPBCAMS assays and CCDC assays performed better (with detection rates of 92 % and 100 %, respectively) than that of the WHO assays (with a detection rate of 60 %), and the ORF 1b gene assay in IPBCAMS assays performed better (with a detection rate of 64 %) than those of the WHO assays and the CCDC assays (with detection rates of 48 % and 20 %, respectively). In conclusion, the N gene assays of CCDC assays and IPBCAMS assays and the ORF 1b gene assay of IPBCAMS assays were recommended for qRT-PCR screening of SARS-CoV-2. |*Real-Time Polymerase Chain Reaction/methods[MESH] |*Reverse Transcriptase Polymerase Chain Reaction/methods[MESH] |COVID-19 Testing/*methods/standards[MESH] |COVID-19/*diagnosis/*virology[MESH] |Humans[MESH] |Reproducibility of Results[MESH] |SARS-CoV-2/*genetics[MESH]Comparison of three TaqMan real-time reverse transcription-PCR assays (epmc).pdf DeepDyve Pubget Overpricing