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10.1016/j.jviromet.2020.114031

http://scihub22266oqcxt.onion/10.1016/j.jviromet.2020.114031
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33275926!7707675!33275926
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suck abstract from ncbi


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pmid33275926      J+Virol+Methods 2021 ; 288 (ä): 114031
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  • Comparison of potency assays to assess SARS-CoV-2 neutralizing antibody capacity in COVID-19 convalescent plasma #MMPMID33275926
  • von Rhein C; Scholz T; Henss L; Kronstein-Wiedemann R; Schwarz T; Rodionov RN; Corman VM; Tonn T; Schnierle BS
  • J Virol Methods 2021[Feb]; 288 (ä): 114031 PMID33275926show ga
  • Convalescent plasma is plasma collected from individuals after resolution of an infection and the development of antibodies. Passive antibody administration by transfusion of convalescent plasma is currently in clinical evaluations to treat COVID-19 patients. The level of neutralizing antibodies vary among convalescent patients and fast and simple methods to identify suitable plasma donations are needed. We compared three methods to determine the SARS-CoV-2 neutralizing activity of human convalescent plasma: life virus neutralization by plaque reduction assay, a lentiviral vector based pseudotype neutralization assay and a competition ELISA-based surrogate virus neutralization assay (sVNT). Neutralization activity correlated among the different assays; however the sVNT assay was overvaluing the low neutralizing plasma. On the other hand, the sVNT assay required the lowest biosafety level, is fast and is sufficient to identify highly neutralizing plasma samples. Though weakly neutralizing samples were more reliable detected by the more challenging lentiviral vector based assays or virus neutralization assays. Spike receptor binding competition assays are suitable to identify highly neutralizing plasma samples under low biosafety requirements. Detailed analysis of in vitro neutralization activity requires more sophisticated methods that have to be performed under higher biosafety levels.
  • |Antibodies, Neutralizing/blood/*immunology[MESH]
  • |Antibodies, Viral/blood/*immunology[MESH]
  • |COVID-19 Serological Testing/*methods/standards[MESH]
  • |COVID-19/blood/*diagnosis/*immunology[MESH]
  • |Cell Line[MESH]
  • |Humans[MESH]
  • |Neutralization Tests/*methods[MESH]


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