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10.1038/s41551-020-00655-z http://scihub22266oqcxt.onion/10.1038/s41551-020-00655-z 33273714!ä!33273714 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Nat+Biomed+Eng 2020 ; 4 (12): 1150-1158 Nephropedia Template TP {{tp|p=33273714|t=2020. Rapid lateral flow immunoassay for the fluorescence detection of SARS-CoV-2 RNA |pdf=|usr=}}{{33273714}} gab.com Text Rapid lateral flow immunoassay for the fluorescence detection of SARS-CoV-2 RNA JO: Nat Biomed Eng http://www.kidney.de/pget.php?&tw=1&pmid=33273714 #FOAvip The coronavirus disease 2019 (COVID-19) pandemic has highlighted the need for rapid and accurate nucleic acid detection at the point of care. Here, we report an amplification-free nucleic acid immunoassay, implemented on a lateral flow strip, for the fluorescence detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA in less than one hour. The assay uses DNA probes that are designed to bind to the conserved open reading frame 1ab (ORF1ab), envelope protein (E) and the nucleocapsid (N) regions of the SARS-CoV-2 genome, and a fluorescent-nanoparticle-labelled monoclonal antibody that binds to double-stranded DNA-RNA hybrids. In a multi-hospital randomized double-blind trial involving 734 samples (593 throat swabs and 141 sputum) provided by 670 individuals, the assay achieved sensitivities of 100% and specificities of 99% for both types of sample (ground truth was determined using quantitative PCR with reverse transcription). The inexpensive amplification-free detection of SARS-CoV-2 RNA should facilitate the rapid diagnosis of COVID-19 at the point of care. Twit Text FOAVip Rapid lateral flow immunoassay for the fluorescence detection of SARS-CoV-2 RNA ????Nat Biomed Eng http://www.kidney.de/pget.php?&tw=1&pmid=33273714 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33273714 #FOAvip English Wikipedia <ref>{{Cite pmid|33273714}}</ref> Rapid lateral flow immunoassay for the fluorescence detection of SARS-CoV-2 RNA #MMPMID33273714 Wang D; He S; Wang X; Yan Y; Liu J; Wu S; Liu S; Lei Y; Chen M; Li L; Zhang J; Zhang L; Hu X; Zheng X; Bai J; Zhang Y; Zhang Y; Song M; Tang Y Nat Biomed Eng 2020[Dec]; 4 (12): 1150-1158 PMID33273714show ga The coronavirus disease 2019 (COVID-19) pandemic has highlighted the need for rapid and accurate nucleic acid detection at the point of care. Here, we report an amplification-free nucleic acid immunoassay, implemented on a lateral flow strip, for the fluorescence detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA in less than one hour. The assay uses DNA probes that are designed to bind to the conserved open reading frame 1ab (ORF1ab), envelope protein (E) and the nucleocapsid (N) regions of the SARS-CoV-2 genome, and a fluorescent-nanoparticle-labelled monoclonal antibody that binds to double-stranded DNA-RNA hybrids. In a multi-hospital randomized double-blind trial involving 734 samples (593 throat swabs and 141 sputum) provided by 670 individuals, the assay achieved sensitivities of 100% and specificities of 99% for both types of sample (ground truth was determined using quantitative PCR with reverse transcription). The inexpensive amplification-free detection of SARS-CoV-2 RNA should facilitate the rapid diagnosis of COVID-19 at the point of care. |Antibodies, Monoclonal/immunology[MESH] |COVID-19/*diagnosis/*immunology/virology[MESH] |Fluorescence[MESH] |Humans[MESH] |Immunoassay/*methods[MESH] |Molecular Diagnostic Techniques/methods[MESH] |Nucleic Acid Amplification Techniques/methods[MESH] |Pandemics/prevention & control[MESH] |Point-of-Care Systems[MESH] |RNA, Viral/*immunology[MESH] |SARS-CoV-2/*immunology[MESH]Rapid lateral flow immunoassay for the fluorescence detection of SARS-(epmc).pdf DeepDyve Pubget Overpricing