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10.3390/ijms21239167 http://scihub22266oqcxt.onion/10.3390/ijms21239167 33271925!7731011!33271925     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Int+J+Mol+Sci 2020 ; 21 (23): ä Nephropedia Template TP {{tp|p=33271925|t=2020. Establishment of Murine Hybridoma Cells Producing Antibodies against Spike Protein of SARS-CoV-2 |pdf=|usr=}}{{33271925}} gab.com Text Establishment of Murine Hybridoma Cells Producing Antibodies against Spike Protein of SARS-CoV-2 JO: Int J Mol Sci http://www.kidney.de/pget.php?&tw=1&pmid=33271925 #FOAvip In 2020 the world faced the pandemic of COVID-19 severe acute respiratory syndrome caused by a new type of coronavirus named SARS-CoV-2. To stop the spread of the disease, it is crucial to create molecular tools allowing the investigation, diagnoses and treatment of COVID-19. One of such tools are monoclonal antibodies (mAbs). In this study we describe the development of hybridoma cells that can produce mouse mAbs against receptor binding domain of SARS-CoV-2 spike (S) protein. These mAbs are able to specifically detect native and denatured S proteins in all tested applications, including immunoblotting, enzyme-linked immunosorbent assay, immunofluorescence staining of cells and immunohistochemical staining of paraffin embedded patients' tissue samples. In addition, we showed that the obtained mAbs can efficiently block SARS-CoV-2 infection in in vitro experiments. Finally, we determined the amino acid sequence of light and heavy chains of the mAbs. This information will allow the use of corresponding peptides to establish genetically engineered therapeutic antibodies. To date multiple mAbs against SARS-CoV-2 proteins have been established, however, bigger sets of various antibodies will allow the detection and neutralization of SARS-CoV-2, even if the virus acquires novel mutations. Twit Text FOAVip Establishment of Murine Hybridoma Cells Producing Antibodies against Spike Protein of SARS-CoV-2 ????Int J Mol Sci http://www.kidney.de/pget.php?&tw=1&pmid=33271925 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33271925 #FOAvip English Wikipedia <ref>{{Cite pmid|33271925}}</ref> Establishment of Murine Hybridoma Cells Producing Antibodies against Spike Protein of SARS-CoV-2 #MMPMID33271925 Antipova NV; Larionova TD; Siniavin AE; Nikiforova MA; Gushchin VA; Babichenko II; Volkov AV; Shakhparonov MI; Pavlyukov MS Int J Mol Sci 2020[Dec]; 21 (23): ä PMID33271925show ga In 2020 the world faced the pandemic of COVID-19 severe acute respiratory syndrome caused by a new type of coronavirus named SARS-CoV-2. To stop the spread of the disease, it is crucial to create molecular tools allowing the investigation, diagnoses and treatment of COVID-19. One of such tools are monoclonal antibodies (mAbs). In this study we describe the development of hybridoma cells that can produce mouse mAbs against receptor binding domain of SARS-CoV-2 spike (S) protein. These mAbs are able to specifically detect native and denatured S proteins in all tested applications, including immunoblotting, enzyme-linked immunosorbent assay, immunofluorescence staining of cells and immunohistochemical staining of paraffin embedded patients' tissue samples. In addition, we showed that the obtained mAbs can efficiently block SARS-CoV-2 infection in in vitro experiments. Finally, we determined the amino acid sequence of light and heavy chains of the mAbs. This information will allow the use of corresponding peptides to establish genetically engineered therapeutic antibodies. To date multiple mAbs against SARS-CoV-2 proteins have been established, however, bigger sets of various antibodies will allow the detection and neutralization of SARS-CoV-2, even if the virus acquires novel mutations. |Amino Acid Sequence[MESH] |Animals[MESH] |Antibodies, Monoclonal/chemistry/immunology/*metabolism[MESH] |Antigen-Antibody Reactions[MESH] |Antigens, Viral/immunology/*metabolism[MESH] |COVID-19/pathology/virology[MESH] |Enzyme-Linked Immunosorbent Assay[MESH] |HEK293 Cells[MESH] |Humans[MESH] |Hybridomas/cytology/metabolism[MESH] |Immunohistochemistry[MESH] |Lung/pathology/virology[MESH] |Mice[MESH] |Mice, Inbred BALB C[MESH] |Protein Domains/immunology[MESH] |Recombinant Proteins/biosynthesis/immunology/isolation & purification[MESH] |SARS-CoV-2/immunology/isolation & purification/*metabolism[MESH]Establishment of Murine Hybridoma Cells Producing Antibodies against S(epmc).pdf DeepDyve Pubget Overpricing