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10.1099/jmm.0.001285

http://scihub22266oqcxt.onion/10.1099/jmm.0.001285
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33270005!8131016!33270005
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suck abstract from ncbi


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pmid33270005      J+Med+Microbiol 2021 ; 70 (2): ä
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  • Detection of SARS-CoV-2 in saliva: implications for specimen transport and storage #MMPMID33270005
  • Williams E; Isles N; Chong B; Bond K; Yoga Y; Druce J; Catton M; Ballard SA; Howden BP; Williamson DA
  • J Med Microbiol 2021[Feb]; 70 (2): ä PMID33270005show ga
  • Saliva has recently been proposed as a suitable specimen for the diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Use of saliva as a diagnostic specimen may present opportunities for SARS-CoV-2 reverse transcription polymerase chain reaction (RT-PCR) testing in remote and low-resource settings. Determining the stability of SARS-CoV-2 RNA in saliva over time is an important step in determining optimal storage and transport times. We undertook an in vitro study to assess whether SARS-CoV-2 could be detected in contrived saliva samples. The contrived saliva samples comprised 10 ml pooled saliva spiked with gamma-irradiated SARS-CoV-2 to achieve a concentration of 2.58x10(4) copies ml SARS-CoV-2, which was subsequently divided into 2 ml aliquots comprising: (i) neat saliva; and a 1 : 1 dilution with (ii) normal saline; (iii) viral transport media, and (iv) liquid Amies medium. Contrived samples were made in quadruplicate, with two samples of each stored at either: (i) room temperature or (ii) 4 degrees C. SARS-CoV-2 was detected in all SARS-CoV-2 spiked samples at time point 0, day 1, 3 and 7 at both storage temperatures using the N gene RT-PCR assay and time point 0, day 1 and day 7 using the Xpert Xpress SARS-CoV-2 (Cepheid, Sunnyvale, USA) RT-PCR assay. The ability to detect SARS-CoV-2 in saliva over a 1 week period is an important finding that presents further opportunities for saliva testing as a diagnostic specimen for the diagnosis of SARS-CoV-2.
  • |COVID-19 Testing/*methods[MESH]
  • |COVID-19/*diagnosis[MESH]
  • |Humans[MESH]
  • |Molecular Diagnostic Techniques[MESH]
  • |RNA, Viral/*analysis[MESH]
  • |Reverse Transcriptase Polymerase Chain Reaction[MESH]
  • |SARS-CoV-2/*genetics/isolation & purification[MESH]
  • |Saliva/*virology[MESH]


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