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10.1021/acssensors.0c01382

http://scihub22266oqcxt.onion/10.1021/acssensors.0c01382
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33267587!ä!33267587

suck abstract from ncbi


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pmid33267587      ACS+Sens 2020 ; 5 (12): 3655-3677
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  • On the Challenges for the Diagnosis of SARS-CoV-2 Based on a Review of Current Methodologies #MMPMID33267587
  • Mattioli IA; Hassan A; Oliveira ON Jr; Crespilho FN
  • ACS Sens 2020[Dec]; 5 (12): 3655-3677 PMID33267587show ga
  • Diagnosis of COVID-19 has been challenging owing to the need for mass testing and for combining distinct types of detection to cover the different stages of the infection. In this review, we have surveyed the most used methodologies for diagnosis of COVID-19, which can be basically categorized into genetic-material detection and immunoassays. Detection of genetic material with real-time polymerase chain reaction (RT-PCR) and similar techniques has been achieved with high accuracy, but these methods are expensive and require time-consuming protocols which are not widely available, especially in less developed countries. Immunoassays for detecting a few antibodies, on the other hand, have been used for rapid, less expensive tests, but their accuracy in diagnosing infected individuals has been limited. We have therefore discussed the strengths and limitations of all of these methodologies, particularly in light of the required combination of tests owing to the long incubation periods. We identified the bottlenecks that prevented mass testing in many countries, and proposed strategies for further action, which are mostly associated with materials science and chemistry. Of special relevance are the methodologies which can be integrated into point-of-care (POC) devices and the use of artificial intelligence that do not require products from a well-developed biotech industry.
  • |*Immunoassay[MESH]
  • |*Real-Time Polymerase Chain Reaction[MESH]
  • |Artificial Intelligence[MESH]
  • |COVID-19/*diagnosis/*virology[MESH]
  • |Humans[MESH]
  • |Point-of-Care Systems[MESH]


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