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10.5858/arpa.2020-0713-SA

http://scihub22266oqcxt.onion/10.5858/arpa.2020-0713-SA
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33264390!ä!33264390

suck abstract from ncbi


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pmid33264390      Arch+Pathol+Lab+Med 2021 ; 145 (4): 415-418
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  • Remote Fingerstick Blood Collection for Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Antibody Testing #MMPMID33264390
  • Garcia-Beltran WF; Miller TE; Kirkpatrick G; Nixon A; Astudillo MG; Yang D; Mahanta LM; Murali M; Dighe AS; Lennerz J; Thierauf J; Naranbhai V; Iafrate AJ
  • Arch Pathol Lab Med 2021[Apr]; 145 (4): 415-418 PMID33264390show ga
  • The rapid worldwide spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection has propelled the rapid development of serologic tests that can detect anti-SARS-CoV-2 antibodies. These have been used for studying the prevalence and spread of infection in different populations, and helping establish a recent diagnosis of coronavirus disease 2019 (COVID-19), and will likely be used to confirm humoral immunity after infection or vaccination. However, nearly all lab-based high-throughput SARS-CoV-2 serologic assays require a serum sample from venous blood draw, limiting their applications and scalability. Here, we present a method that enables large-scale SARS-CoV-2 serologic studies by combining self or office collection of fingerprick blood with a volumetric absorptive microsampling device (Mitra, Neoteryx LLC) with a high-throughput electrochemiluminescence-based SARS-CoV-2 total antibody assay (Roche Elecsys, Roche Diagnostics Inc) that is emergency use authorization approved for use on serum samples and widely used by clinical laboratories around the world. We found that the Roche Elecsys assay has a high dynamic range that allows for accurate detection of SARS-CoV-2 antibodies in serum samples diluted 1:20 as well as contrived dried blood extracts. Extracts of dried blood from Mitra devices acquired in a community seroprevalence study showed near identical sensitivity and specificity in detection of SARS-CoV-2 antibodies compared with neat sera using predefined thresholds for each specimen type. Overall, this study affirms the use of Mitra dried blood collection device with the Roche Elecsys SARS-CoV-2 total antibody assay for remote or at-home testing as well as large-scale community seroprevalence studies.
  • |Antibodies, Viral/*blood[MESH]
  • |Blood Specimen Collection/methods[MESH]
  • |COVID-19 Serological Testing/*methods/statistics & numerical data[MESH]
  • |COVID-19/*diagnosis/epidemiology/immunology[MESH]
  • |Fingers[MESH]
  • |High-Throughput Screening Assays/methods/statistics & numerical data[MESH]
  • |Humans[MESH]
  • |Pandemics[MESH]
  • |Remote Sensing Technology/methods/statistics & numerical data[MESH]
  • |SARS-CoV-2/*immunology[MESH]
  • |Sensitivity and Specificity[MESH]


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