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10.1590/0037-8682-0657-2020 http://scihub22266oqcxt.onion/10.1590/0037-8682-0657-2020 33263691!7723368!33263691     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Rev+Soc+Bras+Med+Trop 2020 ; 53 (ä): e20200657 Nephropedia Template TP {{tp|p=33263691|t=2020. In silico analysis of mismatches in RT-qPCR assays of 177 SARS-CoV-2 sequences from Brazil |pdf=|usr=}}{{33263691}} gab.com Text In silico analysis of mismatches in RT-qPCR assays of 177 SARS-CoV-2 sequences from Brazil JO: Rev Soc Bras Med Trop http://www.kidney.de/pget.php?&tw=1&pmid=33263691 #FOAvip INTRODUCTION: Quantitative reverse transcription polymerase chain reaction (RT-qPCR) can detect the severe acute respiratory syndrome Coronavirus-2 (SARS-CoV-2) in a highly specific manner. However, a decrease in the specificity of PCR assays for their targets may lead to false negative results. METHODS: Here, 177 high-coverage complete SARS-CoV-2 genome sequences from 13 Brazilian states were aligned with 15 WHO recommended PCR assays. RESULTS: Only 3 of the 15 completely aligned to all Brazilian sequences. Ten assays had mismatches in up to 3 sequences and two in many sequences. CONCLUSION: These results should be taken into consideration when using PCR-based diagnostics in Brazil. Twit Text FOAVip In silico analysis of mismatches in RT-qPCR assays of 177 SARS-CoV-2 sequences from Brazil ????Rev Soc Bras Med Trop http://www.kidney.de/pget.php?&tw=1&pmid=33263691 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33263691 #FOAvip English Wikipedia <ref>{{Cite pmid|33263691}}</ref> In silico analysis of mismatches in RT-qPCR assays of 177 SARS-CoV-2 sequences from Brazil #MMPMID33263691 Santos RDS; Bret RSC; Moreira ACOM; Campos AR; Silva ARAE; Lima DM; Tavares KCS Rev Soc Bras Med Trop 2020[]; 53 (ä): e20200657 PMID33263691show ga INTRODUCTION: Quantitative reverse transcription polymerase chain reaction (RT-qPCR) can detect the severe acute respiratory syndrome Coronavirus-2 (SARS-CoV-2) in a highly specific manner. However, a decrease in the specificity of PCR assays for their targets may lead to false negative results. METHODS: Here, 177 high-coverage complete SARS-CoV-2 genome sequences from 13 Brazilian states were aligned with 15 WHO recommended PCR assays. RESULTS: Only 3 of the 15 completely aligned to all Brazilian sequences. Ten assays had mismatches in up to 3 sequences and two in many sequences. CONCLUSION: These results should be taken into consideration when using PCR-based diagnostics in Brazil. |*Genome, Viral[MESH] |Brazil[MESH] |COVID-19/*virology[MESH] |Computer Simulation[MESH] |Humans[MESH] |Pandemics[MESH] |RNA, Viral/genetics[MESH] |Reverse Transcriptase Polymerase Chain Reaction[MESH] |SARS-CoV-2/*genetics[MESH]In silico analysis of mismatches in RT-qPCR assays of 177 SARS-CoV-2 s(epmc).pdf DeepDyve Pubget Overpricing