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10.1038/s41467-020-19843-1 http://scihub22266oqcxt.onion/10.1038/s41467-020-19843-1 33247108!7699647!33247108     free     free     free Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Nat+Commun 2020 ; 11 (1): 6041 Nephropedia Template TP {{tp|p=33247108|t=2020. Nucleocapsid protein of SARS-CoV-2 phase separates into RNA-rich polymerase-containing condensates |pdf=|usr=}}{{33247108}} gab.com Text Nucleocapsid protein of SARS-CoV-2 phase separates into RNA-rich polymerase-containing condensates JO: Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=33247108 #FOAvip The etiologic agent of the Covid-19 pandemic is the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The viral membrane of SARS-CoV-2 surrounds a helical nucleocapsid in which the viral genome is encapsulated by the nucleocapsid protein. The nucleocapsid protein of SARS-CoV-2 is produced at high levels within infected cells, enhances the efficiency of viral RNA transcription, and is essential for viral replication. Here, we show that RNA induces cooperative liquid-liquid phase separation of the SARS-CoV-2 nucleocapsid protein. In agreement with its ability to phase separate in vitro, we show that the protein associates in cells with stress granules, cytoplasmic RNA/protein granules that form through liquid-liquid phase separation and are modulated by viruses to maximize replication efficiency. Liquid-liquid phase separation generates high-density protein/RNA condensates that recruit the RNA-dependent RNA polymerase complex of SARS-CoV-2 providing a mechanism for efficient transcription of viral RNA. Inhibition of RNA-induced phase separation of the nucleocapsid protein by small molecules or biologics thus can interfere with a key step in the SARS-CoV-2 replication cycle. Twit Text FOAVip Nucleocapsid protein of SARS-CoV-2 phase separates into RNA-rich polymerase-containing condensates ????Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=33247108 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33247108 #FOAvip English Wikipedia <ref>{{Cite pmid|33247108}}</ref> Nucleocapsid protein of SARS-CoV-2 phase separates into RNA-rich polymerase-containing condensates #MMPMID33247108 Savastano A; Ibanez de Opakua A; Rankovic M; Zweckstetter M Nat Commun 2020[Nov]; 11 (1): 6041 PMID33247108show ga The etiologic agent of the Covid-19 pandemic is the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The viral membrane of SARS-CoV-2 surrounds a helical nucleocapsid in which the viral genome is encapsulated by the nucleocapsid protein. The nucleocapsid protein of SARS-CoV-2 is produced at high levels within infected cells, enhances the efficiency of viral RNA transcription, and is essential for viral replication. Here, we show that RNA induces cooperative liquid-liquid phase separation of the SARS-CoV-2 nucleocapsid protein. In agreement with its ability to phase separate in vitro, we show that the protein associates in cells with stress granules, cytoplasmic RNA/protein granules that form through liquid-liquid phase separation and are modulated by viruses to maximize replication efficiency. Liquid-liquid phase separation generates high-density protein/RNA condensates that recruit the RNA-dependent RNA polymerase complex of SARS-CoV-2 providing a mechanism for efficient transcription of viral RNA. Inhibition of RNA-induced phase separation of the nucleocapsid protein by small molecules or biologics thus can interfere with a key step in the SARS-CoV-2 replication cycle. |Animals[MESH] |Antiviral Agents/pharmacology/therapeutic use[MESH] |COVID-19 Drug Treatment[MESH] |COVID-19/epidemiology/*virology[MESH] |Coronavirus Nucleocapsid Proteins/antagonists & inhibitors/*metabolism[MESH] |Coronavirus RNA-Dependent RNA Polymerase/metabolism[MESH] |HeLa Cells[MESH] |Humans[MESH] |Insecta[MESH] |Intravital Microscopy[MESH] |Microscopy, Fluorescence[MESH] |Molecular Dynamics Simulation[MESH] |Pandemics/prevention & control[MESH] |Phosphoproteins/antagonists & inhibitors/metabolism[MESH] |RNA, Viral/antagonists & inhibitors/*metabolism[MESH] |SARS-CoV-2/drug effects/*physiology[MESH] |Viral Transcription/drug effects/physiology[MESH]Nucleocapsid protein of SARS-CoV-2 phase separates into RNA-rich poly(epmc).pdf DeepDyve Pubget Overpricing